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  1. 130441
    “…Laser Doppler examination, vascular cast, and immunofluorescence staining were applied to detect the revascularization of lower limbs in mice. Western blot analysis was used to detect the expression of vascular endothelial growth factor (VEGF), interleukin-1beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor- (TNF-) α, heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase quinone-1 (NQO-1), catalase (CAT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphorylated protein kinase B (p-AKT), and phosphorylated glycogen synthase kinase-3 beta (p-GSK3β). …”
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    Buyang Huanwu Decoction Enhances Revascularization via Akt/GSK3β/NRF2 Pathway in Diabetic Hindlimb Ischemia
  2. 130442
    “…The protein synthesis of surface M2 markers was investigated by Western blotting. The statistical analysis was performed by the Wilcoxon t-test. …”
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    CTLA4-Ig treatment induces M1–M2 shift in cultured monocyte-derived macrophages from healthy subjects and rheumatoid arthritis patients
  3. 130443
    “…The expression of vascular endothelial growth factor (VEGF)-A, basic fibroblast growth factor (bFGF), transforming growth factor (TGF)-β1, VEGF-C, claudin-5, occludin, zonula occludens (ZO)-1, fibroblast activation protein, and α-smooth muscle actin was assessed by western blot analysis. The levels of cytokines interleukin (IL)-6, TGF-β1, and VEGF were assessed by enzyme-linked immunosorbent assay. …”
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    BRAF(V600E) mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs
  4. 130444
    “…The specific formation of the ApoE-enriched protein corona around nanoparticles was characterized by Western blotting analysis and LC–MS/MS. The in vitro physicochemical properties and in vivo anti-glioma effects of PTX/Aβ-CN-PMs were also well studied. …”
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    Novel brain-targeted nanomicelles for anti-glioma therapy mediated by the ApoE-enriched protein corona in vivo
  5. 130445
    “…IL-1β-induced IL-6 or IL-8 release levels were determined using enzyme-linked immunosorbent assay, and the involved signalling transduction was explored via Western blotting. RESULTS: Compared with the control groups, the FGF2 protein levels were significantly upregulated in the bronchial epithelium and alveolar areas of clinical asthma samples (6.70 ± 1.79 vs. 16.32 ± 2.40, P = 0.0184; 11.20 ± 2.11 vs. 21.00 ± 3.00, P = 0.033, respectively) and HDM-induced asthmatic mouse lung lysates (1.00 ± 0.15 vs. 5.14 ± 0.42, P < 0.001). …”
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    FGF2 is overexpressed in asthma and promotes airway inflammation through the FGFR/MAPK/NF-κB pathway in airway epithelial cells
  6. 130446
    “…HELA-Exos were identified by transmission electron microscopy, nanoscale flow cytometry, and Western blot analysis. The targeting, killing, and immune activation effects of HELA-Exos were evaluated in vitro. …”
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    Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer
  7. 130447
    Publicado 2022
    “…At the regional level, we estimated the all-age death rate attributable to resistance to be highest in western sub-Saharan Africa, at 27·3 deaths per 100 000 (20·9–35·3), and lowest in Australasia, at 6·5 deaths (4·3–9·4) per 100 000. …”
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    Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis
  8. 130448
    “…Black tea accounts for over 90% of all teas sold in western countries. The world’s top-grade black teas include Qi Men black in China, Darjeeling and Assam black tea in India, and Uva black tea in Sri Lanka. …”
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    Tea and tea drinking: China’s outstanding contributions to the mankind
  9. 130449
    “…Confocal microscopy was performed to examine RIN3-dependent subcellular localization of BIN1V1 and BIN1V9. Western blot analysis was performed to assess the effects of RIN3 and BIN1V1/BIN1V9 on β-secretase mediated processing of APP. …”
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    The neuronal-specific isoform of BIN1 regulates β-secretase cleavage of APP and Aβ generation in a RIN3-dependent manner
  10. 130450
    “…The tumors were collected and evaluated: the tumor volume and growth rate were gauged to evaluate tumor growth; hematoxylin-eosin staining was performed to estimate histopathological changes; immunofluorescence (IF) was performed to detect the expression of CD31, α-SMA and collagen IV; transmission electron microscopy (TEM) was conducted to observe the morphological structure of vascular cells; enzyme-linked immunosorbent assay (ELISA) was performed to measure the levels of secreted HIF-1α and TNF-α; reverse transcription-polymerase chain reaction (RT-qPCR) was performed to measure the mRNA expression of HIF-1α, TNF-α, VEGF and MMP-9; and Western blot (WB) was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and MAPK signaling pathways. …”
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    Frankincense myrrh attenuates hepatocellular carcinoma by regulating tumor blood vessel development through multiple epidermal growth factor receptor-mediated signaling pathways
  11. 130451
    “…No human PK ethnicity difference was identified between Chinese and western patients. CONCLUSIONS: With an encouraging ORR, the MARCH study has demonstrated that selinexor combined with low-dose dexamethasone (Sd) delivers meaningful clinical benefit to Chinese patients with RRMM, including triple-class refractory patients. …”
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    Selinexor plus low-dose dexamethasone in Chinese patients with relapsed/refractory multiple myeloma previously treated with an immunomodulatory agent and a proteasome inhibitor (MARCH): a phase II, single-arm study
  12. 130452
    “…Expression of GalT, hCD55, and hCD39 was analyzed by FACS and western blotting. Human complement-mediated cytotoxicity and human antibody binding assays were conducted on peripheral blood mononuclear cells (PBMCs) and red blood cells (RBCs), and deposition of C3 by incubation with human complement serum and platelet aggregation were analyzed in GGTA1 knock-out (GTKO)/CD55/CD39 pig cells. …”
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    A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model
  13. 130453
    “…Quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting, immunohistochemical staining and immunofluorescence were used to detect protein localization and expression. …”
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    miR-188-3p-targeted regulation of ATG7 affects cell autophagy in patients with nonobstructive azoospermia
  14. 130454
    “…BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is the commonest liver condition in the western world and is directly linked to obesity and the metabolic syndrome. …”
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    Evaluating future risk of NAFLD in adolescents: a prediction and decision curve analysis
  15. 130455
    “…Technology is developing rapidly in Japan, Taiwan, South Korea and Singapore, as many researchers and educators obtain master's and doctoral degrees from western countries and then return to Asian countries to teach new technology, which brings rich human resources. …”
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    REVIEW ON THE HISTORY AND EMOTIONAL BEHAVIOR CHANGES OF R&D HUMAN RESOURCES IN JAPAN, SOUTH KOREA AND SINGAPORE
  16. 130456
    por Yang, Louie H., Swan, Karen, Bastin, Eric, Aguilar, Jessica, Cenzer, Meredith, Codd, Andrew, Gonzalez, Natalie, Hayes, Tracie, Higgins, August, Lor, Xang, Macharaga, Chido, McMunn, Marshall, Oto, Kenya, Winarto, Nicholas, Wong, Darren, Yang, Tabatha, Afridi, Numan, Aguilar, Sarah, Allison, Amelia, Ambrose‐Winters, Arden, Amescua, Edwin, Apse, Mattias, Avoce, Nancy, Bastin, Kirstin, Bolander, Emily, Burroughs, Jessica, Cabrera, Cristian, Candy, Madeline, Cavett, Ariana, Cavett, Melina, Chang, Lemuel, Claret, Miles, Coleman, Delaney, Concha, Jacob, Danzer, Paxson, DaRosa, Joe, Dufresne, Audrey, Duisenberg, Claire, Earl, Allyson, Eckey, Emily, English, Maddie, Espejo, Alexander, Faith, Erika, Fang, Amy, Gamez, Alejandro, Garcini, Jackelin, Garcini, Julie, Gilbert‐Igelsrud, Giancarlo, Goedde‐Matthews, Kelly, Grahn, Sarah, Guerra, Paloma, Guerra, Vanessa, Hagedorn, Madison, Hall, Katie, Hall, Griffin, Hammond, Jake, Hargadon, Cody, Henley, Victoria, Hinesley, Sarah, Jacobs, Celeste, Johnson, Camille, Johnson, Tattiana, Johnson, Zachary, Juchau, Emma, Kaplan, Celeste, Katznelson, Andrew, Keeley, Ronja, Kubik, Tatum, Lam, Theodore, Lansing, Chalinee, Lara, Andrea, Le, Vivian, Lee, Breana, Lee, Kyra, Lemmo, Maddy, Lucio, Scott, Luo, Angela, Malakzay, Salman, Mangney, Luke, Martin, Joseph, Matern, Wade, McConnell, Byron, McHale, Maya, McIsaac, Giulia, McLennan, Carolanne, Milbrodt, Stephanie, Mohammed, Mohammed, Mooney‐McCarthy, Morgan, Morgan, Laura, Mullin, Clare, Needles, Sarah, Nunes, Kayla, O'Keeffe, Fiona, O'Keeffe, Olivia, Osgood, Geoffrey, Padilla, Jessica, Padilla, Sabina, Palacio, Isabella, Panelli, Verio, Paulson, Kendal, Pearson, Jace, Perez, Tate, Phrakonekham, Brenda, Pitsillides, Iason, Preisler, Alex, Preisler, Nicholas, Ramirez, Hailey, Ransom, Sylvan, Renaud, Camille, Rocha, Tracy, Saris, Haley, Schemrich, Ryan, Schoenig, Lyla, Sears, Sophia, Sharma, Anand, Siu, Jessica, Spangler, Maddie, Standefer, Shaili, Strickland, Kelly, Stritzel, Makaila, Talbert, Emily, Taylor, Sage, Thomsen, Emma, Toups, Katrina, Tran, Kyle, Tran, Hong, Tuqiri, Maraia, Valdes, Sara, VanVorhis, George, Vue, Sandy, Wallace, Shauna, Whipple, Johnna, Yang, Paja, Ye, Meg, Yo, David, Zeng, Yichao
    Publicado 2022
    “…Here, we document seasonal changes in milkweed (Asclepias fascicularis)–monarch (Danaus plexippus) interactions with high resolution throughout the last three breeding seasons prior to a precipitous single‐year decline in the western monarch population. Our results show early‐ and late‐season windows of opportunity for monarch recruitment that were constrained by different combinations of factors. …”
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    Different factors limit early‐ and late‐season windows of opportunity for monarch development
  17. 130457
    “…The visit included completion of a patient-reported outcome, the Western Ontario Rotator Cuff score (WORC) and clinical assessment. …”
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    Paper 65: Arthroscopic rotator cuff repair with and without acromioplasty in the treatment of full-thickness rotator cuff tears: long term follow-up of a multicenter, randomized controlled trial
  18. 130458
    “…Expression and clinicopathologic significance of α5-nAChR and PD-L1 were detected by immunohistochemistry in a tissue microarray. α5-nAChR regulated LUAD cell immune escape by targeting the STAT3/Jab1-PD-L1 signalling by Western-blotting and ChIP in vitro. We used T cell coculture, flow cytometry, ELISA, CCK8 assay and crystal violet staining to detect the expression of regulatory T cell (Tregs), IFN-γ, IL-2 and the ability of T cell-mediated tumour cell killing respectively. …”
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    Alpha5 nicotinic acetylcholine receptor mediated immune escape of lung adenocarcinoma via STAT3/Jab1-PD-L1 signalling
  19. 130459
    “…The protein levels of VEGF-C/VEGFR-3-mediated downstream signals and the NF-κB pathway were assayed by western blot. Faecal microbiota was measured by 16S rRNA sequencing. …”
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    ADSCs stimulated by VEGF-C alleviate intestinal inflammation via dual mechanisms of enhancing lymphatic drainage by a VEGF-C/VEGFR-3-dependent mechanism and inhibiting the NF-κB pathway by the secretome
  20. 130460
    “…Then, ELISA was adopted for quantifying immunoglobulin E (IgE), interleukin (IL)-4, IL-6, and histamine (HA), and nasal mucosa tissues were treated by HE and TUNEL staining to observing their histopathological manifestations. PCR and western blot (WB) were adopted for quantifying GzmB and miR-378a-3p. …”
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    Influences of miR-378a-3p on the Pathogenesis of Allergic Rhinitis via GzmB-Mediated Inflammatory Reaction
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