“…Finally, use of the model to explore situations with limited data is illustrated by simulating the kinetics and disposition of Pb during acute Pb poisoning and chelation therapy in a child.…”
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“…WRKY and GCM1 are metal chelating DNA-binding domains (DBD) which share a four stranded fold. …”
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“…In all the predicted low-energy complexes, oxygen atoms of correolide chelate a K+ ion. Correolide-sensing residues known from mutational analysis along with the ligand-bound K+ ion provide major contributions to the ligand-binding energy. …”
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“…Furthermore, polyphenolic antioxidants such as quercetin, caffeic acid or purpurogallin, the radical trap Tempol or the iron chelator desferrioxamine prevented tirapazamine-mediated cytotoxicity. …”
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“…In other control experiments, either the Ca ionophore A23187 or the Ca chelator EGTA was added to the incubation media; electron-dense deposits were very rarely observed within the intraterminal organelles of these saponin-treated synaptosomes. …”
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“…Co-electrophoresis of purified calmodulin and radioactively labeled SCb proteins in two dimensional polyacrylamide gel electrophoresis (PAGE) demonstrated the identity of the heat-stable SCb protein and calmodulin on the basis of pI, molecular weight, and anomalous migration in the presence of Ca2+-chelating agents. No proteins co-migrating with calmodulin in two-dimensional PAGE could be detected among the proteins of slow component a (SCa; 0.3 mm/d, microtubules and neurofilaments) or fast component (FC; 250 mm/d, membrane-associated proteins). …”
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“…Increasing the concentration of salt or adding ion chelators solubilized some of the 36-kdalton protein that otherwise was pelletable with high g forces. …”
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“…This major Arg- Gly-Asp-directed receptor on M21 cells could be metabolically labeled with 45Ca2+. Chelation of this ion with EDTA caused the dissociation of GD2 from the receptor and rendered the remaining glycoprotein incapable of binding to an Arg-Gly-Asp-containing peptide. …”
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“…Here, we show that the GPI-anchored CD59 molecule on Jurkat T cells is internalized after cross-linking, a process inhibited by nystatin, a sterol chelating agent. Clustered CD59 molecules mostly accumulate in non-coated invaginations of the lymphocyte membrane before endocytosis, in marked contrast with the pattern of CD3-TCR internalization. …”
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“…Recovery of infectious A9 virus was also possible with a chelating compound. Coxsackie A9 virus could also be dissociated by means of low pH after adsorption onto susceptible cells for 2 minutes at 1°C., but not after 1 hour incubation at 37°C., indicating that viral host cell activity may be required in order for irreversible enterovirus attachment to take place.…”
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“…Both magnesium and calcium ions were required for full adhesiveness; calcium ions alone were unable to restore adhesiveness to PMN from blood which had been treated with a chelating resin to remove divalent cations. The adhesiveness of the PMN was independent of cyanide and dinitrophenol, but was almost completely eliminated by iodoacetamide. …”
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“…Spreading is abolished by chelation of intracellular Ca(2+). Demonstration that adhesion of platelets to collagen via α(2)β(1) generates intracellular signals provides a new insight into the mechanisms that control thrombus formation and may explain the unstable nature of β(1)-deficient thrombi and why loss of the GPVI–FcR γ-chain complex has a relatively minor effect on bleeding.…”
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“…The inhibitory action of heparin is not due to chelation of magnesium. Heparin is most active in inhibiting convertase formation on cellular intermediates formed with the lowest C3b input and developed with the highest B concentration, thereby suggesting the receptor site for B on C3b as the point of heparin action. …”
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“…The anticoagulant effect of HDA was inhibited by freshly prepared hematin, fresh Sn-protoporphyrin, imidazole, or the iron chelator desferrioxamine. Ferrioxamine did not inhibit HDA, and inhibition by imidazole was reversed with ferric citrate, suggesting a role for iron in the mechanism of HDA activity. …”
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“…The sequences encoding human and fungal P(2) protein were subcloned, expressed in Escherichia coli as His(6)-tagged fusion proteins, and purified by Ni(2+)–chelate affinity chromatography. Both recombinant P(2) proteins were recognized by IgE antibodies from allergic individuals sensitized to the A. fumigatus P(2) protein and elicited strong type 1–specific skin reactions in these individuals. …”
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“…Conditions which change the affinities, capacities, and mobilities of plasma membrane ligands that bind calcium or cause extracellular chelation of calcium are capable of altering the rate of calcium transport.…”
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“…The elevated [Ca2+]i was unrelated to previously observed changes in intracellular pH, because chelating the Ca2+ in the medium failed to inhibit the elevation of pHi that occurred during cell spreading. …”
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“…Stimulation of sarcoplasmic reticulum Ca release by Mg reduction of caffeine was studied in situ, to characterize further the Ca2+ dependence observed previously with stimulation by Cl ion. 45Ca efflux and isometric force were measured simultaneously at 19 degrees C in frog skeletal muscle fibers skinned by microdissection; EGTA was added to chelate myofilament space Ca either before or after the stimulus. …”
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“…This response was followed for 55 s, with 5 mM EGTA added to chelate myofilament space (MFS) Ca either (a) after relaxation, (b) near the peak of the force spike, or (c) before or with the stimulus. …”
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“…Moreover, A- 23187 rapidly releases previously accumulated Ca from these (nonmitochondrial) storage sites, whereas the Ca chelator, EGTA, does not. The proteolytic enzyme, trypsin, spares the mitochondria but inactivates the nonmitochondrial Ca uptake mechanism. …”
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