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“…In this proof-of-concept study, a homogeneous and isothermal photoacoustic biosensor is demonstrated for rapid molecular amplification and detection of a synthetic DNA corresponding to SARS-CoV-2 spike N501Y. Branched rolling circle amplification produces single-stranded amplicons that can aggregate detection probe-modified AuNPs, which induces a strong photoacoustic signal at 640 nm due to both the surface plasmon resonance shift and the size-dependent effect of laser-induced nanobubbles, achieving a sub-femtomolar detection limit within a total assay time of 80 min. …”
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“…RESULTS: A retrospective study using human genomic DNA samples of known genotype was performed for four different clinically relevant mutations: Factor V Leiden, Factor II prothrombin, and two hemochromatosis mutations, C282Y and H63D. …”
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“…The K(+) current of homozygous (cir/cir) mice was more sensitive to TEA in the 1 to 10 mM range, while the 4-AP sensitivities were not different between the two genotypes. Removal of external Ca(2+) did not affect the K(+) currents in either genotype, indicating that the higher sensitivity of K(+) current to TEA in the homozygous (cir/cir) mice was not due to an early expression of Ca(2+) activated K(+) channels. …”
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“…Simulated osteotomies were conducted on the 3D models of glenoids at two cutting locations, expressed as clock face times (2:30–4:20; 1:30–5:00). …”
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“…A higher number of individual-rein asymmetry variables—particularly when the limb that contributed to movement asymmetry on the straight was on the inside of the circle—were affected by speed (nine variables, all p ≤ 0.047) and surface (three variables, all p ≤ 0.037) compared with average-rein asymmetry variables (two for speed, all p ≤ 0.003; two for surface, all p ≤ 0.046). …”
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“…The Theil index was close to 0, indicating that the equity of government health expenditure in the Chengdu–Chongqing economic circle was generally good. 2. The health level of residents in the Chengdu–Chongqing economic circle had improved compared to before. 3. …”
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“…After RCA, HBV cccDNA was further amplified by a pair of selective primers labeled digoxigenin that target the gap region between the two direct repeat regions (DR1 and DR2) of the virus via IS-PCR. …”
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“…Precommunicating segment of the posterior cerebral arteries (P1) was hypoplastic bilaterally in 3 (2%), unilaterally in 14 (4%), and AcomA was hypoplastic in 91 (25%). …”
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“…Data were collected at baseline (T0), immediately after the intervention at week 8 (T1), and at week 12 (T2) at the post-intervention and follow-up session. …”
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“…The morphological pattern of the circles was recorded. The prepared circles were then put between two glass plates and tightly compressed. …”
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“…Primary collateral grades (PCG) at baseline based on the integrity of Willis' circle were categorized into three degrees. The favorable outcomes, measured by modified Rankin scale (mRS), were defined as ≤2 at 90 days. …”
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“…We found that plasmids of the rolling-circle Rep_2 family (to which pMV158 belongs) are more frequently found in Lactobacillales than in any other bacterial order, whereas Rep_1 initiators seemed to prefer hosts included in the Bacillales order. …”
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“…METHODS: This study took a participatory design by using research circle (RC) methodology. Two RC groups were created, one with occupational therapists and one with citizens with obesity. …”
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“…The results show that (1) the overall trend of coordinated development of green economy in the Chengdu-Chongqing economic circle is enhanced; (2) the pulling force includes the similarity of economic agglomeration and regional openness, the resonance force includes the similarity of traffic and industrial structure, the pushing force comes from the central city; (3) in the urban agglomeration with double centers, the cooperation and competition between two “pole” cities may coexist.…”
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“…The Demographic and Health Survey carried out in 2001 revealed that, in Kita Circle, in the Kayes region, only 13% of children aged 12 to 23 months had received all the EPI vaccinations. …”
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