“…Upon blue light activation, a covalent bond is formed between VVD residue Cys108 and its cofactor flavin adenine dinucleotide(FAD), and prompts VVD switching from Dark state to Light state with significant conformational deviation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Pretreatment with an antioxidant or cytochrome P450 (CYP), flavin monooxygenase (FMO), or peroxidase inhibitor was used in some experiments. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Thus AzmE, a putative S-adenosyl methionine-dependent methyl transferase, is responsible for late-stage O-methylation reactions in azolemycin biosynthesis and AzmF, a putative flavin-dependent monooxygenase, catalyzes oxidation of the N-terminal amino group in an azolemycin precursor to the corresponding oxime. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Further experiments with the recombinant maize enzyme obtained from yeast expression system showed that rather than the catalytic activity of the enzyme itself, a non-enzymatic flavin induced photoisomerization is responsible for the observed zeatin cis–trans interconversion in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…For a deeper insight into these biophysical mechanisms in cryptochrome, we had introduced a flavin-tryptophan dyad (F10T). Here we present the magnetic field dependence of (1)H photo-CIDNP NMR on F10T and a theoretical model for low-field photo-CIDNP of F10T. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In this work, we report the spectroelectrochemical detection of individual molecules of the redox-active, fluorogenic molecule flavin mononucleotide (FMN) freely diffusing in solution. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…CO expression is induced and CO protein is stabilized by FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1) in the late afternoon, while CO is degraded by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) during the night. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The VAO flavoprotein family consists mostly of oxidoreductases harboring a covalently linked flavin cofactor. The linkage can be either monocovalent at position 8 with a histidine or tyrosine or bicovalent at position 8 with a histidine and at position 6 with a cysteine. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The ltbsg1 gene in mutant revealed a single nucleotide substitution (G-A) in the exon 2 of LOC_Os10g25780, causing an amino acid variation (Glycine-Arginine) in the FAD (Flavin-adenine dinucleotide)-binding domain of delta (24)-sterol reductase, which was involved in the brassinosteroid (BR) biosynthesis. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Membrane localization of one such photoreceptor, BcLOV4 from Botrytis cinerea, is directly coupled to its flavin photocycle, and is mediated by a polybasic amphipathic helix in the linker region between the LOV sensor and its C-terminal domain of unknown function (DUF), as revealed through a combination of bioinformatics, computational protein modeling, structure–function studies, and optogenetic assays in yeast and mammalian cell line expression systems. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…One class of enzyme for which a universal, operationally simple ultra-high throughput (>10(6) variants per day) assay is not available is flavin adenine dinucleotide (FAD) dependent oxidases. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Domain-swapped, trimeric STEAP4 orients NADPH bound to a cytosolic domain onto axially aligned flavin-adenine dinucleotide (FAD) and a single b-type heme that cross the transmembrane-domain to enable electron transfer. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This new sugar oxidase was composed of a single polypeptide chain with a molecular mass of 47.2 kDa and was found to contain 2.0 zinc ions per mole of enzyme but no flavin adenine dinucleotide or heme. This enzyme was stable in the pH range 5.5–9.0, with an optimal reaction pH of 7.5. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We show that the synthetic capacity is significantly improved when two pathways work simultaneously comparing to each individual pathway. Next, we engineer flavin-dependent monooxygenase HpaBC for tyrosol hydroxylase, tyramine hydroxylase, and promiscuous hydroxylase active on both tyrosol and tyramine using directed divergent evolution strategy. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…HmfF belongs to an uncharacterized branch of the prenylated flavin (prFMN) dependent UbiD family of reversible (de)carboxylases and is proposed to convert 2,5-furandicarboxylic acid (FDCA) to furoic acid in vivo. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The 12-oxo-phytodienoic acid reductases (OPRs), which belong to the old yellow enzyme (OYE) family, are flavin mononucleotide (FMN)-dependent oxidoreductases with critical functions in plants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Comparative analysis showed that the genome of strain DM2 encodes some strain-specific proteins in comparison with B. subtilis subsp. subtilis str. 168, such as carboxymuconolactone decarboxylase family protein, gfo/Idh/MocA family oxidoreductases, GlsB/YeaQ/YmgE family stress response membrane protein, HlyC/CorC family transporters, LLM class flavin-dependent oxidoreductase, and LPXTG cell wall anchor domain-containing protein. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This project focused on the study of LSD2, a flavin-dependent histone demethylase found to be overexpressed in numerous cancers. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Complex I deficiency is a mitochondrial disorder usually associated with early-onset severe multisystem disorders characterized by highly variable clinical manifestations. Flavin adenine dinucleotide (FAD)-dependent oxidoreductase domain-containing protein 1 (FOXRED1) is a complex I assembly factor. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we developed a genetically encoded dual-function probe, named p H and redox-sensitive fluorescent protein (pHaROS), for simultaneous real-time detection of changes in redox potential and pH in living cells. pHaROS consists of the Arabidopsis flavin mononucleotide-binding fluorescent protein iLOV and an mKATE variant, mBeRFP. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso