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5961por Zhou, Cong, Zhang, Hui, Xu, Maosuo, Liu, Yajuan, Yuan, Baoyu, Lin, Yong, Shen, Fang“…The virulence potential of the strains was evaluated using Galleria mellonella larvae infection model, serum resistance assay, capsular polysaccharide quantification, and biofilm formation assay. …”
Publicado 2023
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5962por Lam, Margaret MC, Seemann, Torsten, Tobias, Nicholas J, Chen, Honglei, Haring, Volker, Moore, Robert J, Ballard, Susan, Grayson, Lindsay M, Johnson, Paul DR, Howden, Benjamin P, Stinear, Timothy P“…Additional phenotype comparisons between ST17 and ST203 isolates showed that while there were no differences in biofilm-formation and killing of Galleria mellonella, ST203 isolates grew significantly faster and out-competed ST17 isolates in growth assays. …”
Publicado 2013
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5963por Sherry, Leighann, Rajendran, Ranjith, Lappin, David F, Borghi, Elisa, Perdoni, Federica, Falleni, Monica, Tosi, Delfina, Smith, Karen, Williams, Craig, Jones, Brian, Nile, Chris J, Ramage, Gordon“…HBF isolates were more resistance to amphotericin B (AMB) treatment than LBF, but not voriconazole (VRZ). In a Galleria mellonella model of infection HBF mortality was significantly increased in comparison to LBF. …”
Publicado 2014
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5964por Navarro-Arias, María J., Defosse, Tatiana A., Dementhon, Karine, Csonka, Katalin, Mellado-Mojica, Erika, Dias Valério, Aline, González-Hernández, Roberto J., Courdavault, Vincent, Clastre, Marc, Hernández, Nahúm V., Pérez-García, Luis A., Singh, Dhirendra K., Vizler, Csaba, Gácser, Attila, Almeida, Ricardo S., Noël, Thierry, López, Mercedes G., Papon, Nicolas, Mora-Montes, Héctor M.“…Accordingly, the null mutant showed inability to kill larvae in the Galleria mellonella infection model. This study thus demonstrates that mannans are relevant for the C. guilliermondii-host interaction, with an atypical role for O-linked mannans.…”
Publicado 2016
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5965por Roszniowski, Bartosz, Latka, Agnieszka, Maciejewska, Barbara, Vandenheuvel, Dieter, Olszak, Tomasz, Briers, Yves, Holt, Giles S., Valvano, Miguel A., Lavigne, Rob, Smith, Darren L., Drulis-Kawa, Zuzanna“…The lysogenic bacterial mutants did not exhibit any significant changes in virulence compared to wild-type host strain when tested in the Galleria mellonella moth wax model. Moreover, AP3 treatment of larvae infected with B. cenocepacia revealed a significant increase (P < 0.0001) in larvae survival in comparison to AP3-untreated infected larvae. …”
Publicado 2016
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5966por Trujillo-Esquivel, Elías, Martínez-Álvarez, José A., Clavijo-Giraldo, Diana M., Hernández, Nahúm V., Flores-Martínez, Alberto, Ponce-Noyola, Patricia, Mora-Montes, Héctor M.“…Furthermore, it displayed not transcriptional changes when S. schenckii infected larvae of Galleria mellonella or interacted with immune cells. …”
Publicado 2017
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5967por Vicente, Vania A., Weiss, Vinícius A., Bombassaro, Amanda, Moreno, Leandro F., Costa, Flávia F., Raittz, Roberto T., Leão, Aniele C., Gomes, Renata R., Bocca, Anamelia L., Fornari, Gheniffer, de Castro, Raffael J. A., Sun, Jiufeng, Faoro, Helisson, Tadra-Sfeir, Michelle Z., Baura, Valter, Balsanelli, Eduardo, Almeida, Sandro R., Dos Santos, Suelen S., Teixeira, Marcus de Melo, Soares Felipe, Maria S., do Nascimento, Mariana Machado Fidelis, Pedrosa, Fabio O., Steffens, Maria B., Attili-Angelis, Derlene, Najafzadeh, Mohammad J., Queiroz-Telles, Flávio, Souza, Emanuel M., De Hoog, Sybren“…Virulence was tested in the Galleria mellonella model and immunological assays were performed in order to support this hypothesis. …”
Publicado 2017
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5968por Geng, Jianing, Huang, Szu-Chuan, Chen, Yueh-Ying, Chiu, Cheng-Hsun, Hu, Songnian, Chen, Yi-Ywan M.“…Inactivation of codY reduced the glycolytic capacity and the viability of S. salivarius at pH 5.5 or in the presence of H(2)O(2). Studies using the Galleria mellonella larva model showed that CodY was essential for the toxicity generated from S. salivarius infection, suggesting that CodY regulation was critical for immune evasion and systemic infections. …”
Publicado 2018
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5969por Aneja, Babita, Azam, Mudsser, Alam, Shadab, Perwez, Ahmad, Maguire, Ronan, Yadava, Umesh, Kavanagh, Kevin, Daniliuc, Constantin G., Rizvi, M. Moshahid A., Haq, Qazi Mohd. Rizwanul, Abid, Mohammad“…Treatment of larvae of Galleria mellonella (an in vivo model for antimicrobial studies) with 5e and 5u did not cause an alteration in the hemocyte density, thereby indicating lack of an immune response, and were nontoxic up to a concentration of 2.5 mg/mL.…”
Publicado 2018
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5970por Marini, Emanuela, Magi, Gloria, Ferretti, Gianna, Bacchetti, Tiziana, Giuliani, Angelica, Pugnaloni, Armanda, Rippo, Maria Rita, Facinelli, Bruna“…The ability to form biofilm and to invade Caco-2 cells was also significantly reduced. Galleria mellonella larvae infected with L. monocytogenes grown in presence of sublethal concentrations of the essential oil showed much higher survival rates compared with controls, suggesting that the extract inhibited tissue invasion. …”
Publicado 2018
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5971“…Virulence was evaluated in Galleria mellonella larvae by injecting 10(7) cells/larvae. …”
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5972por Fernandez-Garcia, Laura, Ambroa, Antón, Blasco, Lucia, Bleriot, Ines, López, Maria, Alvarez-Marin, Rocio, Fernández-Cuenca, Felipe, Martinez-Martinez, Luis, Vila, Jordi, Rodríguez-Baño, Jesús, Garnacho-Montero, Jose, Cisneros, Jose Miguel, Pascual, Alvaro, Pachón, Jeronimo, Bou, German, Smani, Younes, Tomás, Maria“…However, in the A. baumannii strains isolated from patients with non-bacteraemic pneumonia, aidA gene expression was regulated by stressors such as 3-oxo-C12-HSL and H(2)O(2). In an in vivo Galleria mellonella model of A. baumannii infection, the A. baumannii ATCC 17978 strain was associated with higher mortality (100% at 24 h) than the mutant, abaI-deficient, strain (carrying a synthetase enzyme of Acyl homoserine lactone molecules) (70% at 24 h). …”
Publicado 2018
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5973“…Further experiments demonstrated that this strain was classified as a nonbiofilm producer and serum sensitivity (grade 1) and killed only 30% of Galleria mellonella inoculated with 1×10(6) colony-forming unit of the specimen within 48 hours, suggesting relatively low virulence. …”
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5974por Xie, Tian, Wang, Yulong, Yu, Deshui, Zhang, Qilin, Zhang, Tingting, Wang, Zhangxun, Huang, Bo“…We also demonstrated that the mean lethal time to death (LT(50)) of ΔMrSVP significantly increased relative to the wild type in insect bioassays (both topical inoculation and injection) involving Galleria mellonella. Moreover, similar rates of appressorium formation between ΔMrSVP and the wild type—and the significantly different expression of virulence-related genes such as acid trehalase and sucrose nonfermenting protein kinase in the haemocoel after injection—revealed that MrSVP is required for virulence in the insect haemocoel. …”
Publicado 2019
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5975por Lee, Muyoung, Pinto, Naina Adren, Kim, Chan Yeong, Yang, Sunmo, D’Souza, Roshan, Yong, Dongeun, Lee, Insuk“…Using complementation assays with the top candidate genes, we were able to validate a novel gene that negatively regulated carbapenem resistance and four novel genes that positively regulated virulence in Galleria mellonella larvae. Therefore, our study demonstrated the feasibility of network-based identification of genes required for antibiotic resistance and virulence of human-pathogenic bacteria. …”
Publicado 2019
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5976por de Melo, Amanda Teixeira, Martho, Kevin Felipe, Roberto, Thiago Nunes, Nishiduka, Erika S., Machado, Joel, Brustolini, Otávio J. B., Tashima, Alexandre K., Vasconcelos, Ana Tereza, Vallim, Marcelo A., Pascon, Renata C.“…In addition, we found that Cys3 is required for virulence in Galleria mellonella animal model.…”
Publicado 2019
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5977por Alnezary, Faris S, Rashid, Tasnuva, Begum, Khurshida, Carlson, Travis J, Gonzales-Luna, Anne J, Alam, M Jahangir, Garey, Kevin W“…BACKGROUND: Antimicrobials disrupt the gut microbiota by reducing gut microbiome diversity and quantity. Galleria mellonella provides an invertebrate model that is inexpensive, easy to maintain, and does not require specialized equipment. …”
Publicado 2019
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5978por Warnock, Neil D., Cox, Deborah, McCoy, Ciaran, Morris, Robert, Dalzell, Johnathan J.“…S. carpocapsae Breton displays increased chemotaxis toward the laboratory host Galleria mellonella, relative to the other strains. …”
Publicado 2019
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5979por Singulani, Junya de Lacorte, Galeane, Mariana Cristina, Ramos, Marina Dorisse, Gomes, Paulo César, dos Santos, Claudia Tavares, de Souza, Bibiana Monson, Palma, Mario Sergio, Fusco Almeida, Ana Marisa, Mendes Giannini, Maria José Soares“…The need for new antifungals has therefore prompted a screen for inhibitory peptides, which have multiple mechanisms of action. The honeycomb moth Galleria mellonella has been widely used as a model system for evaluating efficacy of antifungal agents. …”
Publicado 2019
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5980por Ding, Lingchi, Yang, Zhiqiang, Lu, Junguo, Ma, Lichao, Liu, Ying, Wu, Xiaoyan, Yao, Weidong, Zhang, Xiaodong, Zhu, Kui“…Microbiological methods, antimicrobial susceptibility tests, pulsed-field gel electrophoresis (PFGE), polymerase chain reaction (PCR) assays, Sanger sequencing and Galleria mellonella larvae infection model were used in this study. …”
Publicado 2020
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