“…We then confirmed the effect of Pluronic F68 (PF-68) at 3.0 g/L on HEK293 cell growth and Ad-HER production in shake flasks and a 2 L benchtop bioreactor. RESULTS: The optimized process was scale-up to a 2 L benchtop bioreactor with the PATFP perfusion system, which yielded cell density of 7.4 × 10(6) cells/mL and Ad-HER titer of 9.8 × 10(9) IFU/mL at 2 dpi, comparable to the bioreactor with a ATF2 system. …”
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“…Oxygen also resulted in improved bile tolerance and boosted 5′nucleotidase activity (370 U/L vs. 240 U/L in N(2) sparged conditions) but lower tolerance to acidic conditions compared bacteria grown under complete anaerobic conditions which survived up to 90 min of exposure at pH 2. …”
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“…Microbial degradation of petroleum hydrocarbons is a crucial process for the clean-up of oil-contaminated environments. Cycloclasticus spp. are well-known polycyclic aromatic hydrocarbon (PAH) degraders that possess PAH-degradation marker genes including rhd3α, rhd2α, and pahE. …”
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“…The most consistent pairwise effect on rheumatoid arthritis was found between two markers within MAP3K7IP2/SUMO4 on 6q25.1, although LR and RFs assigned different significance levels. …”
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“…RESULTS: Seven strains belonging to different taxonomic groups isolated from the seawater-brine interface of four hypersaline lakes were grown at medium salinity and then incubated in the brines. …”
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“…Se toxicity increased in cultures grown under sulfur deficient conditions. We selected three strains of Scenedesmus quadricauda specifically resistant to high concentrations of inorganic selenium added as selenite (Na(2)SeO(3)) – strain SeIV, selenate (Na(2)SeO(4)) – strain SeVI or both – strain SeIV+VI. …”
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“…Diuretic activity had a rapid onset (1(st) h), peaked at 2(nd) h and maintained up to 4(th) h (except the low dose). …”
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“…No difference in tumor volume or syrinx size was observed between Groups 2 and 3. Five tumors in Group 1 were resected during follow-up because symptoms had developed or the tumor had significantly grown. …”
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“…We conducted a fully factorial experiment in which plants were grown at ambient and/or elevated levels of O(3) and CO(2), to simulate present and projected levels of both gases and their potential interactive effects. …”
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“…Er(3+)/Yb(3+)/Pr(3+): SrGdGa(3)O(7) crystal was firstly grown by Czochralski method. Detailed spectroscopic analyses of Er(3+)/Yb(3+)/Pr(3+): SrGdGa(3)O(7) were carried out. …”
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“…Concrete admixtures Ca(NO(3))(2) and Ca(HCOO)(2) were used as nutrients. The enrichment culture, grown as granules (0.5–2 mm) consisting of 70% biomass and 30% inorganic salts were added into mortar without any additional protection. …”
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“…It showed high phenol degrading efficiency up to 1117 mg L(–1) within 60 h by the secretion of catechol 1,2-dioxygenase via ortho intradial pathway. …”
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“…Here, the impact of FA as a foliar application at 0, 0.1, and 0.2 mM was studied on snap bean seedlings grown under non-saline and salinity stress (50 mM NaCl) conditions. …”
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“…In FTIR spectra, the asymmetric stretching vibration signal of the internal (PO(2))(−) units, which is present in the Ca-polyP coacervate formed at pH 7, disappears in the NP formed at pH 10 using the conventional method (dropping of a CaCl(2) solution into a Na-polyP solution). …”
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“…Here we show that recently designed molecular phototransducer can be used to stimulate C2C12 skeletal muscle cells, properly grown to exhibit collective behaviour. …”
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“…By linking the DOTA chelating agent to position 8 of the deamino derivative of Lys(8)-vasotocin (dLVT), we obtained a new compound (DOTA-dLVT) with the following characteristics: (1) it forms a monomeric and stable compound that binds to OTR with an affinity comparable to that of the endogenous OT ligand; (2) it is characterised by a very good selectivity profile for the human OTR, with a low affinity binding to the closely related V1a, V1b and V2 vasopressin receptor subtypes; (3) it induces rapid and persistent receptor internalisation and (4) when radiolabelled, [(111)In]-DOTA-dLVT is efficiently and selectively taken up by OTR-positive tumours grown in mice. …”
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“…The as-grown BP films exhibit excellent electrical properties, with a field-effect and Hall mobility of over 1200 cm(2)V(−1)s(−1) and 1400 cm(2)V(−1)s(−1) at room temperature, respectively, comparable to those exfoliated from BP bulk crystals. …”
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“…In this paper, we present the first experimental evidence of the piezoelectric nature of struvite (MgNH(4)PO(4)·6H(2)O). Using a single diffusion gel growth technique, we have grown struvite crystals in the form of plane parallel plates. …”
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“…Inhibition of L. plantarum growth was investigated and compared with other LAB at a pH range of 6.2–5.0. Interestingly, valinomycin inhibited L. plantarum growth from pH 6.2 to 5.0. …”
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“…In contrast, certain genes involved in metabolism and cell growth, such as porphobilinogen deaminase and tyrosine phosphatase, and one SICAvar gene, were significantly up-regulated in vitro. CONCLUSIONS: This study demonstrates how gene expression in P. knowlesi blood-stage parasites can differ dramatically depending on whether the parasites are grown in vivo, with only one cycle of development ex vivo, or as an adapted isolate in long-term in vitro culture. …”
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