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  1. 14721
    por Hamarsheh, Omar, Amro, Ahmad
    Publicado 2011
    “…The enrichment methods used previously for finding new microsatellite loci in sand flies remain laborious and time consuming; in silico mining, which includes retrieval and screening of microsatellites from large amounts of sequence data from sequence data bases using microsatellite search tools can yield many new candidate markers. …”
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    Characterization of simple sequence repeats (SSRs) from Phlebotomus papatasi (Diptera: Psychodidae) expressed sequence tags (ESTs)
  2. 14722
    “…Although random mutagenesis can be used to generate TS mutants, the procedure is laborious and unfeasible in multicellular organisms. …”
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    Neighborhood Properties Are Important Determinants of Temperature Sensitive Mutations
  3. 14723
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    Total hip replacement in young adults with hip dysplasia: Age at diagnosis, previous treatment, quality of life, and validation of diagnoses reported to the Norwegian Arthroplasty Register between 1987 and 2007
  4. 14724
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    In situ fixation of slipped capital femoral epiphysis with Steinmann pins: 67 patients followed for 2–16 years
  5. 14725
    “…Our relatively poor understanding of the functions of non-coding genomic sequence, and the slow and laborious process of experimental validation of the functional significance of human regulatory variants, limits our ability to fully benefit from this information in our efforts to comprehend human disease. …”
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    Modelling Human Regulatory Variation in Mouse: Finding the Function in Genome-Wide Association Studies and Whole-Genome Sequencing
  6. 14726
    “…Yersinia pestis dissemination in a host is usually studied by enumerating bacteria in the tissues of animals sacrificed at different times. This laborious methodology gives only snapshots of the infection, as the infectious process is not synchronized. …”
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    Imaging of Bubonic Plague Dynamics by In Vivo Tracking of Bioluminescent Yersinia pestis
  7. 14727
    “…However, the experimental discovery and validation of bona fide granzyme B substrates require time consuming and laborious efforts. As such, computational methods for the prediction of substrates would be immensely helpful. …”
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    In silico prediction of the granzyme B degradome
  8. 14728
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    Total hip arthroplasty in young adults, with focus on Perthes' disease and slipped capital femoral epiphysis: Follow-up of 540 subjects reported to the Norwegian Arthroplasty Register during 1987–2007
  9. 14729
    “…So far, these trial-and-error techniques have proved laborious and sensitive to process parameters, while there has been no attempt to address the problem by applying Synthetic Biology principles and methods, such as integration of standardized parts in novel synthetic circuits. …”
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    A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli
  10. 14730
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    Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development
  11. 14731
    “…However, the refolding process is time-consuming and laborious due to the variability of the behavior of each protein and requires a great deal of trial-and-error to achieve success. …”
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    An Automatic Refolding Apparatus for Preparative-Scale Protein Production
  12. 14732
    “…The traditional lifespan assay relies on manual micro-manipulation to remove daughter cells from the mother, which is laborious, time consuming, and does not allow long term tracking with high resolution microscopy. …”
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    Single Cell Analysis of Yeast Replicative Aging Using a New Generation of Microfluidic Device
  13. 14733
    “…However, current methods of administering questionnaires are cumbersome, expensive, and laborious to accurately administer, track and score. eZhengtricity is a simple Google Documents based workflow, coupled with customized Excel formulas, that allow researchers to create, collect and score confidential health questionnaires that are globally accessible, with minimal setup time and maintenance. …”
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    448 Ezhengtricity 2010: a Free Novel Way to Confidentially Administer, Track, Receive and Score Medical Questionnaires Instantly
  14. 14734
    “…Auxotrophic mutants of Aspergillus can be isolated in the presence of counter-selective compounds, but the process is laborious. We developed a method to enable reversible impairment of the ku80 gene (Aaku80) in the imperfect fungus Aspergillus aculeatus. …”
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    Reversible impairment of the ku80 gene by a recyclable marker in Aspergillus aculeatus
  15. 14735
    “…The use of highly conserved but abundant anchor sequences reduces the need for laborious screening for usable primers that result in polymorphic fingerprints, and appears to be the main reason for ease of transferability of our protocol between distantly related taxa.…”
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    Towards a Transferable and Cost-Effective Plant AFLP Protocol
  16. 14736
    “…It is tailored to facilitate laborious tasks such as the interactive annotation of gene function, the discovery of regulatory elements, or the sequence-based reconstruction of gene regulatory networks. …”
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    MGcV: the microbial genomic context viewer for comparative genome analysis
  17. 14737
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    RNA global alignment in the joint sequence–structure space using elastic shape analysis
  18. 14738
    “…Although these two populations can be distinguished from one another using a bioassay, the work is laborious and time-consuming. In this study, a fragment of the intergenic spacer (IGS) region of ribosomal DNA from an Fo biological control agent, Fo366, was PCR-amplified with published general primers, FIGS11/FIGS12 and sequenced. …”
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    Modified Primers for the Identification of Nonpathogenic Fusarium oxysporum Isolates That Have Biological Control Potential against Fusarium Wilt of Cucumber in Taiwan
  19. 14739
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    Safety and efficacy of CYT387, a JAK1 and JAK2 inhibitor, in myelofibrosis
  20. 14740
    “…A variety of NMR chemical shift prediction approaches have been presented in the past, but nearly all of these rely on laborious manual data set preparation and the training itself is not automatized, making retraining the model, e.g., if new data is made available, or testing new models a time-consuming manual chore. …”
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    NightShift: NMR shift inference by general hybrid model training - a framework for NMR chemical shift prediction
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