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  1. 15121
    por Zhang, Xue, Xu, Jin, Xiao, Wang-xin
    Publicado 2013
    “…BACKGROUND: Experimental methods for the identification of essential proteins are always costly, time-consuming, and laborious. It is a challenging task to find protein essentiality only through experiments. …”
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    A New Method for the Discovery of Essential Proteins
  2. 15122
    “…BACKGROUND: Despite the importance of abnormalities in lipoprotein metabolism in clinical canine medicine, the fact that most previously used methods for lipoprotein profiling are rather laborious and time-consuming has been a major obstacle to the wide clinical application and use of lipoprotein profiling in this species. …”
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    Novel lipoprotein density profiling in healthy dogs of various breeds, healthy miniature schnauzers, and miniature schnauzers with hyperlipidemia
  3. 15123
    por Davis, George E, Lowell, Walter E
    Publicado 2013
    “…In the late 19th and early 20th centuries ethnic minorities had poorer nutrition, laborious work, and leaner bodies, and in that environment a calorie-conserving epigenome was a survival advantage. …”
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    Variation in ultraviolet radiation and diabetes: evidence of an epigenetic effect that modulates diabetics’ lifespan
  4. 15124
    “…The identification of these regulators can be laborious and is usually carried out one factor at a time. …”
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    A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing
  5. 15125
    “…Pathogenic mutations can occur in any of the 100 or more disease genes identified so far, making molecular diagnosis a rather laborious process. In this work we explored the use of whole exome sequencing (WES) as a tool for identification of RD mutations, with the aim of assessing its applicability in a diagnostic context. …”
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    Exome Sequencing of Index Patients with Retinal Dystrophies as a Tool for Molecular Diagnosis
  6. 15126
    “…As time-consuming and laborious test crosses with a CMS tester are necessary to identify maintainer lines, development of a marker-assisted selection method for the rf gene (the nonrestoring allele of restorer-of-fertility locus) is highly desirable for sugar-beet breeding. …”
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    Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L.): development of molecular markers for the maintainer genotype
  7. 15127
    “…There are no virus-free cultivars and garlic-infecting viruses are difficult to purify, which make specific antibody production very laborious. Since high quality antisera against plant viruses are important tools for serological detection, we have developed a method to express and purify full-length plant virus coat proteins using baculovirus expression system and insects as bioreactors. …”
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    A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification
  8. 15128
    por Ritchie, Scott A, Devine, Gregor J
    Publicado 2013
    “…Resin-based metofluthrin emanators show great potential as a replacement for labor intensive IRS for dengue vector control.…”
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    Confusion, knock-down and kill of Aedes aegypti using metofluthrin in domestic settings: a powerful tool to prevent dengue transmission?
  9. 15129
    “…However, experimental identification of PPIs is a laborious and error-prone process, and current methods of PPI prediction tend to be highly conservative or require large amounts of functional data that may not be available for newly-sequenced organisms. …”
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    Predicting whole genome protein interaction networks from primary sequence data in model and non-model organisms using ENTS
  10. 15130
    “…MLST is regarded the gold standard for phylogenetic classification of bacteria, but is rather laborious to carry out in many settings. Our data suggest that MLVA can predict the MLST-type with high accuracy, and even higher when studying the clonal complex level. …”
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    Four Genotyping Schemes for Phylogenetic Analysis of Pseudomonas aeruginosa: Comparison of Their Congruence with Multi-Locus Sequence Typing
  11. 15131
    “…Cowie et al. (2010) and Almeida and Lebold (2010) investigated FOG as the changes in velocity profiles of PD gait, as patients walked through a doorway with variable width. …”
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    A computational model of altered gait patterns in parkinson's disease patients negotiating narrow doorways
  12. 15132
    “…Manually updating annotations is therefore highly laborious. This prompted us to look for open-source software applications that could facilitate automatic updating of annotations by mapping between available metabolite identifiers. …”
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    Comparative evaluation of open source software for mapping between metabolite identifiers in metabolic network reconstructions: application to Recon 2
  13. 15133
    “…This new adoptive immunotherapy should be an alternative to the laborious development of MoAb drugs against cancers for which no effective treatments currently exist.…”
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    A Novel and Effective Cancer Immunotherapy Mouse Model Using Antigen-Specific B Cells Selected In Vitro
  14. 15134
    “…The conventional drug susceptibility testing (DST) methods for detection of drug-resistant M.tuberculosis are laborious and cannot provide the rapid detection for clinical practice. …”
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    Pyrosequencing for rapid detection of Tuberculosis resistance in clinical isolates and Sputum samples from re-treatment Pulmonary Tuberculosis patients
  15. 15135
    “…Cell wall compositional analysis for biomass characterization is laborious and time consuming. In order to characterize biomass fast and efficiently, several high through-put technologies have been successfully developed. …”
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    NIR and Py-mbms coupled with multivariate data analysis as a high-throughput biomass characterization technique: a review
  16. 15136
    “…In equivocal cases, oral food challenges (OFCs) are required. However, OFCs are laborious and not without risk; thus, a test that could accurately diagnose peanut allergy and reduce the need for OFCs is desirable. …”
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    Basophil activation test discriminates between allergy and tolerance in peanut-sensitized children
  17. 15137
    por Shin, Jae Ho, Lee, Sang Yup
    Publicado 2014
    “…While the initial approaches involved random mutagenesis for increased tolerance to ARG and consequently higher ARG titer, it is laborious and often leads to unwanted phenotypes, such as retarded growth. …”
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    Metabolic engineering of microorganisms for the production of L-arginine and its derivatives
  18. 15138
    “…We investigated spatio-temporal patterns in microbial community composition and diversity, macrofaunal abundance and their sediment reworking activity, and N-cycling in seven subtidal stations in the Southern North Sea. …”
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    The Link between Microbial Diversity and Nitrogen Cycling in Marine Sediments Is Modulated by Macrofaunal Bioturbation
  19. 15139
    “…However, they are too spacious, laborious and expensive to be operated in larger number in parallel. …”
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    Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors
  20. 15140
    “…We report the first case in otolaryngology literature of a patient with Crouzon syndrome with late CSF rhinorrhea and encephalocele formation after previous LeFort III facial advancement surgery. OBJECTIVES: Describe the case of a patient with Crouzon syndrome who presented with CSF rhinorrhea and encephaloceles as complications of Le Fort III facial advancement surgery. …”
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    Bilateral meningoencephaloceles with cerebrospinal fluid rhinorrhea after facial advancement in the Crouzon syndrome
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