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  1. 15161
    por Zhu, Yanan, Ouyang, Qi, Mao, Youdong
    Publicado 2017
    “…Particle extraction from experimental micrographs thus can be laborious and presents a major practical bottleneck in cryo-EM structural determination. …”
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  2. 15162
  3. 15163
  4. 15164
    “…The nested RT-PCR sequencing assay was the most sensitive but the most laborious assay for detecting macrolide-resistance-associated mutations. …”
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  5. 15165
    “…Spike and grain traits are critical determinants of final yield and grain uniformity a commercially desired trait, but their analysis is laborious and often requires destructive harvest. One of the current challenges is to develop an accurate, non-destructive method for spike and grain trait analysis capable of handling large populations. …”
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  6. 15166
    “…However, AMPs are currently prepared using processes which are often laborious, expensive and of low-yield, thus hindering their research and application. …”
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  7. 15167
    “…Quantitative viral outgrowth assay (QVOA), the reference standard to quantify the amount of replication-competent virus, has several limitations, as it is laborious, expensive, and unable to robustly reactivate every single integrated provirus. …”
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  8. 15168
    “…High basal expression level and low induction ratio can be removed using genetic engineering techniques, although this process is often laborious and time-consuming. RESULTS: In order to facilitate optimization process for inducible promoters, we developed a simple method based on a conditional toxin expression which we abbreviate as toxin expression control strategy (TECS). …”
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  9. 15169
    “…Its widespread use in clinical practice is hampered by the laborious process of flow rate (Q) calculation. OBJETIVE: This study proposes a less burdensome, alternative method of Q calculation to be incorporated in the original formula of EOA(proj) and measures the agreement between the new proposed method of EOA(proj) calculation and the original one. …”
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  10. 15170
    “…Despite falling costs this is still an expensive and laborious technique, particularly when studying large cohorts. …”
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  11. 15171
    “…However, the treatment planning and irradiation are laborious and time‐consuming. We herein present the multijaw‐size concave arc technique (MCAT) as a total scalp irradiation method that overcomes these problems. …”
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  12. 15172
    “…By reducing the demand to conduct laborious and cost-intensive lab-scale bioreactor cultivations during process development, this study will contribute to an accelerated development of protein production processes. …”
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  13. 15173
    “…Zooplankton dominate the abundance and biomass of multicellular animals in pelagic marine environments; however, traditional methods to characterize zooplankton communities are invasive and laborious. This study compares zooplankton taxonomic composition revealed through metabarcoding of the cytochrome oxidase I (COI) and 18S rRNA genes to traditional morphological identification by microscopy. …”
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  14. 15174
    por Lee, Ingoo, Nam, Hojung
    Publicado 2018
    “…However, identifying drug-target interactions via in-vitro, in-vivo experiments are very laborious, time-consuming. Thus, predicting drug-target interactions by using computational approaches is a good alternative. …”
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  15. 15175
  16. 15176
    “…Enzymatic and cellular methods are employed before such sequencing to help in the selection of the gene defects to be sought, but this is time-consuming and laborious. Furthermore some cases remain undiagnosed because no biochemical methods have been available to test for cobalamin absorption and transport defects. …”
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  17. 15177
    “…Methods used to assess compound efficacy on intracellular amastigotes often rely on microscopy-based assays. These are laborious, require specialist equipment and can only determine parasite burden, not parasite viability. …”
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  18. 15178
    “…A number of diagnostic tests have to be performed in order to arrive at a probable pathogen causing AES thus making it a very time consuming, laborious and expensive. The problem is further compounded by the lack of availability of sufficient volume of Cerebrospinal fluid (CSF). …”
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  19. 15179
    “…Proximal remote sensing systems depending on spectral reflectance measurements and image analysis can acquire timely information to make real-time management decisions compared to laborious destructive measurements. There is a need to make nitrogen management decisions at early development stages of cereals when the first top-dressing is made. …”
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  20. 15180
    “…CONCLUSION: Utilizing the proposed model in this paper, while reducing the setup costs and speeding up the launch of various remote monitoring systems, many rework in the implementation of these systems is also reduced.…”
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