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  1. 15641
    “…However, detection of metabolic resistance is not trivial and relies on laborious bioassays, unspecific biochemical methods, or sophisticated and expensive molecular approaches using transcriptomics. …”
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    Rapid multiplex gene expression assays for monitoring metabolic resistance in the major malaria vector Anopheles gambiae
  2. 15642
    “…Although the standard method of manually scoring micronucleated (MNed) cells by microscope is a reliable and standard method, it is laborious and time-consuming. A high-throughput assay system for detecting MN cells automatically has long been desired in the fields of pharmaceutical development or environmental risk monitoring. …”
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    High-content imaging analyses of γH2AX-foci and micronuclei in TK6 cells elucidated genotoxicity of chemicals and their clastogenic/aneugenic mode of action
  3. 15643
    “…Most of the established immobilization methods require case-to-case optimization, which is laborious and time-consuming. Often, (chromatographic) enzyme purification is required and stable immobilization usually includes additional cross-linking or adsorption steps. …”
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    Tailoring the properties of (catalytically)-active inclusion bodies
  4. 15644
    “…All post-Zanclean Otodus megalodon occurrences from the eastern North Pacific exhibit clear evidence of reworking or lack reliable provenance; the youngest reliable records of Otodus megalodon are early Pliocene, suggesting an extinction at the early-late Pliocene boundary (∼3.6 Ma), corresponding with youngest occurrences of Otodus megalodon in Japan, the North Atlantic, and Mediterranean. …”
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    The Early Pliocene extinction of the mega-toothed shark Otodus megalodon: a view from the eastern North Pacific
  5. 15645
    “…Integration of the Rock-Eval, organic petrology and biomarkers data reveal that the kerogen constituents of the source rocks in Araromi well are mainly Type II/III, III and IV with a high amount of inertinite constituents suggesting they have been reworked. Type II and II/III kerogen derived from marine algae are better preserved in the deeper part of Gbekebo well located more southerly in the basin than in the Araromi well. …”
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    The assessment of potential source rocks of Maastrichtian Araromi formation in Araromi and Gbekebo wells Dahomey Basin, southwestern Nigeria
  6. 15646
    “…Manual identification of gene families is highly time-consuming and laborious, requiring an iterative process of manual and computational analysis to identify members of a given family, typically combining numerous BLAST searches and manually cleaning data. …”
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    geneHummus: an R package to define gene families and their expression in legumes and beyond
  7. 15647
    “…No further confirmatory tests are performed since the gold standard (sodium dodecylsulfate polyacrylamide gel electrophoresis, SDS–PAGE) is laborious and time consuming. We propose a test for rapid and easy detection of porin loss by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). …”
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    2155. Accelerated Confirmation of Porin Loss in Carbapenem-Resistant Enterobacterales: A MALDI-TOF Mass Spectrometry-Based Approach
  8. 15648
    “…BACKGROUND: Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious. Such a method is still used to measure potency of live Oral Poliovirus Vaccine during vaccine development and production and in some clinical trials. …”
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    Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine
  9. 15649
    “…In Switzerland, despite the presence of competent vectors, routine surveillance of arboviruses in mosquitoes is not being carried out, mainly due to the high costs associated with the need of a constant cold chain and laborious processing of thousands of mosquitoes. An alternative approach is using honey-baited nucleic acid preserving cards (FTA cards) to collect mosquito saliva that may be analysed for arboviruses. …”
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    Evaluation of honey-baited FTA cards in combination with different mosquito traps in an area of low arbovirus prevalence
  10. 15650
    “…However, as levels of antibodies to multiple serotypes are monitored in thousands of samples, a need for a less laborious and more flexible method has evolved. Fluorescent-bead-based multiplex immunoassays (MIAs) are suitable for this purpose. …”
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    Multilaboratory Comparison of Pneumococcal Multiplex Immunoassays Used in Immunosurveillance of Streptococcus pneumoniae across Europe
  11. 15651
    “…Extraction of NPs, formed within the cells or cell-wall, is laborious, time-consuming and are not cost effective. …”
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    Green pyomelanin-mediated synthesis of gold nanoparticles: modelling and design, physico-chemical and biological characteristics
  12. 15652
    “…However, existing methods for predicting falls are laborious and costly. OBJECTIVE: The objective of this study is to verify whether hospital inpatient falls can be predicted through the analysis of a single input—unstructured nursing records obtained from Japanese electronic medical records (EMRs)—using a natural language processing (NLP) algorithm and machine learning. …”
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    Predicting Inpatient Falls Using Natural Language Processing of Nursing Records Obtained From Japanese Electronic Medical Records: Case-Control Study
  13. 15653
    “…Alternatives to derive the blood time-activity curve (BTAC) non-invasively are either reliant on large vessels in the field of view or are laborious to implement and analyze as well as being prone to many processing errors. …”
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    Simulation study of a coincidence detection system for non-invasive determination of arterial blood time-activity curve measurements
  14. 15654
    “…Diagnostic techniques such as a 24 hour urine collection or saline suppression test (SST) can be laborious for both patients and staff. Our group previously showed that human hair cortisol measurements correlated with urine and serum cortisol levels in patients with endogenous cortisol excess. …”
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    SAT-543 Human Hair Aldosterone Measurements for Evaluation of Primary Aldosteronism
  15. 15655
    “…Answers were compared with Best Operational Practice (BOP), and reworked into a Weighted Index of Compliance (WIC) per section. …”
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    A new protocol for monitoring operational outcomes of environmental management in commercial forestry plantations
  16. 15656
    “…Using creatinine to assess the glomerular filtration rate (GFR) is notoriously inaccurate, and determination of the true GFR, e.g., using inulin or iohexol, is laborious and not feasible in daily practice. Proenkephalin (PENK) is a novel candidate biomarker for kidney function that is filtrated in the glomerulus, has shown to represent steady-state GFR in patients with different severities of renal insufficiency. …”
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    Proenkephalin Compared to Conventional Methods to Assess Kidney Function in Critically Ill Sepsis Patients
  17. 15657
    “…However, tumor vaccines loaded with a single tumor antigen may induce immunosuppression and immune evasion, while identifying tumor-specific antigens can require expensive and laborious procedures. Therefore, the use of whole tumor cell antigens are currently considered to be promising, potentially effective, methods. …”
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    Immunotherapy of Tumor RNA-Loaded Lipid Nanoparticles Against Hepatocellular Carcinoma
  18. 15658
    “…As the drug discovery is a costly, time-consuming, laborious, and highly risk process, the novel approach of drug repositioning is employed to increases the success rate of drug development. …”
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    Drug Repurposing Strategy (DRS): Emerging Approach to Identify Potential Therapeutics for Treatment of Novel Coronavirus Infection
  19. 15659
    “…However, the determination of pollen transfer in the field is complex and laborious. We developed a field experiment in a pollinator-dependent crop and used high throughput RNA sequencing (RNA-seq) to quantify pollen flow by measuring changes in gene expression between pollination treatments across different apple (Malus domestica Borkh.) cultivars. …”
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    Using RNA-seq to characterize pollen–stigma interactions for pollination studies
  20. 15660
    “…Despite advances in experimental technology, it is still expensive, laborious, and time-consuming to determine protein–protein interactions (PPIs), and there is a strong demand for effective bioinformatics approaches to identify potential PPIs. …”
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    Performance improvement for a 2D convolutional neural network by using SSC encoding on protein–protein interaction tasks
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