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111981por Zhang, Fan, Huang, Li-Yu, Zhang, Fan, Ali, Jauhar, Cruz, Casiana Vera, Zhuo, Da-Long, Du, Zheng-Lin, Li, Zhi-kang, Zhou, Yong-Li“…In this study, the transcriptome profiling of a rice line carrying Xa39 and its parents at the early stage of Xoo infection were investigated. …”
Publicado 2015
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111982por He, Zhenggui, Zhang, Lujia, Mao, Youzhi, Gu, Jingchao, Pan, Qi, Zhou, Sixing, Gao, Bei, Wei, Dongzhi“…RESULTS: A novel fungal glucoamylase (RpGla) gene encoding a putative protein of 512 amino acid residues was cloned from Rhizomucor pusillus. …”
Publicado 2014
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111983por Chaudhari, Atul A, Jasper, Shanese L, Dosunmu, Ejovwoke, Miller, Michael E, Arnold, Robert D, Singh, Shree R, Pillai, Shreekumar“…MATERIALS AND METHODS: In this study we created novel pegylated SWCNTS-Ag (pSWCNTs-Ag), and employed 3 eukaryotic cell lines to evaluate their cytotoxicity as compared to plain SWCNTS-Ag. …”
Publicado 2015
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111984Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiencypor Peng, Hao-Yang, Qi, Yi-Ping, Lee, Jinwook, Yang, Lin-Tong, Guo, Peng, Jiang, Huan-Xin, Chen, Li-Song“…To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism. …”
Publicado 2015
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111985“…Ten patients with UFF (net ultrafiltration <400 mL/4 h on 3.86% glucose) were treated with a combination of glycerol and icodextrin with or without amino acid-based dialysis solutions for 3 months. …”
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111986“…Gene ontology analysis identified enrichment of gene sets involved in single-organism metabolic processes, amino acid metabolic processes, ATP and metal ion binding, glycolysis, glutamine metabolic processes, and cobalt and iron ion binding. …”
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111987por Gold, Nicholas D., Gowen, Christopher M., Lussier, Francois-Xavier, Cautha, Sarat C., Mahadevan, Radhakrishnan, Martin, Vincent J. J.“…We evaluated these strategies and provide recommendations for further improvement of aromatic amino acid biosynthesis in S. cerevisiae. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0252-2) contains supplementary material, which is available to authorized users.…”
Publicado 2015
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111988por CHEN, ZE-TAN, ZHAO, WEI, QU, SONG, LI, LING, LU, XIAO-DI, SU, FANG, LIANG, ZHONG-GUO, GUO, SI-YAN, ZHU, XIAO-DONG“…CNE-2 cells were treated with 10 Gy IR in the presence or absence of the AMPK activator 5-amino-1-β-D-ribofuranosyl-1H-imidazole-4-carboxamide (AICAR). …”
Publicado 2015
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111989por Tai, Hui Fong, Foo, Hooi Ling, Abdul Rahim, Raha, Loh, Teck Chewn, Abdullah, Mohd. Puad, Yoshinobu, Kimura“…UL4-plw locus that contained three ORFs were arranged in one operon and possessed remarkable amino acid sequence of LMG2379-plw locus, suggesting it was highly conserved. …”
Publicado 2015
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111990por Struyfs, Hanne, Niemantsverdriet, Ellis, Goossens, Joery, Fransen, Erik, Martin, Jean-Jacques, De Deyn, Peter P., Engelborghs, Sebastiaan“…CSF concentrations of amyloid-β peptide of 42 amino acids (Aβ(1–42)), total tau protein (T-tau), and P-tau(181P) were determined with single analyte ELISA-kits (INNOTEST(®), Fujirebio, Ghent, Belgium). …”
Publicado 2015
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111991por Yong, Sheila T., Nguyen, Hoai-Nghia, Choi, Jae H., Bortner, Carl D., Williams, Jason, Pulloor, Niyas K., Krishnan, Manoj N., Shears, Stephen B.“…RESULTS: Multiple sequence alignments revealed that the metazoan PPIP5K2 family contains a candidate NLS within a strikingly well-conserved 63 amino-acid domain. By analyzing the distribution of hPPIP5K2-GFP in HEK293T cells with the techniques of confocal microscopy and imaging flow cytometry, we found that a distinct pool of hPPIP5K2 is present in the nucleus. …”
Publicado 2015
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111992por Li, Xiuli, Gu, Fang, Niu, Chenguang, Wang, Yuanfen, Liu, Zhongyu, Li, Na, Pan, Bing, He, Dan, Kong, Jian, Zhang, Shaobo, Wang, Xu, Yao, Yuanqing, Zheng, Lemin“…METHORDS: VEGF111b mRNA was detected in ovarian cancer cell lines SKOV3 and OVCAR3 by RT-PCR. Western blot was used to detect VEGF111b and VEGF165b protein in the CMs and lysates of OVCAR3 cells. …”
Publicado 2015
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111993por Ruggieri, Alessandra, Brancati, Francesco, Zanotti, Simona, Maggi, Lorenzo, Pasanisi, Maria Barbara, Saredi, Simona, Terracciano, Chiara, Antozzi, Carlo, D′Apice, Maria Rosaria, Sangiuolo, Federica, Novelli, Giuseppe, Marshall, Christian R., Scherer, Stephen W., Morandi, Lucia, Federici, Luca, Massa, Roberto, Mora, Marina, Minassian, Berge A.“…The protein aggregation myopathy Limb-Girdle Muscular Dystrophy 1D (LGMD1D) is caused by mutations of amino acids Phe89 or Phe93 of DNAJB6, a co-chaperone of the HSP70 anti-aggregation protein. …”
Publicado 2015
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111994por Norkiene, Milda, Stonyte, Jomante, Ziogiene, Danguole, Mazeike, Egle, Sasnauskas, Kestutis, Gedvilaite, Alma“…This translation resulted in a 364-amino acid-long VP1 protein, which efficiently self-assembled into typical PyV VLPs. …”
Publicado 2015
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111995por Iloki-Assanga, Simon B., Lewis-Luján, Lidianys M., Lara-Espinoza, Claudia L., Gil-Salido, Armida A., Fernandez-Angulo, Daniela, Rubio-Pino, Jose L., Haines, David D.“…RESULTS: Phytochemical screening of each plant extract evaluated revealed the following major results: (1) No evidence of alkaloids for each of the extraction phases tested was detected in the hexanic, ethanolic, or aqueous phases of Bucida buceras and Phoradendron californicum (oak and mesquite); (2) Analysis of the hexane phase of B. buceras and P. californicum (mesquite) extracts revealed the presence of carotenes, triterpenes/steroids, and lactonic groups; (3) Analysis of the ethanol and aqueous extraction phases for both plants revealed the presence of a diverse range of compounds, including tripterpenes/steroids, lactonics groups, saponins, phenols/tannins, amines and/or amino acids, and flavonoids/anthocyanins; and (4) The highest total phenolic and flavonoid content were observed in P. californicum (oak): 523.886 ± 51.457 µg GAE/mg extract and 409.651 ± 23.091 µg/mg of extract for methanol and aqueous fractions, respectively. …”
Publicado 2015
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111996por Garg, Anchal, Agrawal, Lalit, Misra, Rajesh Chandra, Sharma, Shubha, Ghosh, Sumit“…ApCPS1, ApCPS2 and ApCPS3 encode for 832-, 817- and 797- amino acids proteins of 55–63 % identity, respectively. …”
Publicado 2015
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111997por Klammert, Uwe, Mueller, Thomas D., Hellmann, Tina V., Wuerzler, Kristian K., Kotzsch, Alexander, Schliermann, Anna, Schmitz, Werner, Kuebler, Alexander C., Sebald, Walter, Nickel, Joachim“…GDF-5, in contrast to BMP-2, shows preferential binding to the isoform BMPR-IB, which is abrogated by a single amino acid (A57R) substitution. The resulting variant, GDF-5 R57A, represents a “BMP-2 mimic” with respect to BMP receptor binding. …”
Publicado 2015
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111998por Mikkelsen, Ulla Ramer, Dideriksen, Kasper, Andersen, Mads Bisgaard, Boesen, Anders, Malmgaard-Clausen, Nikolai Mølkjær, Sørensen, Inge Juul, Schjerling, Peter, Kjær, Michael, Holm, Lars“…Plasma levels of C-reactive protein (CRP), interleukin (IL)-6 and tumor necrosis factor (TNF)-α were measured using enzyme-linked immunosorbent assay (ELISA) in resting blood samples obtained on two separate days. …”
Publicado 2015
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111999por Zhang, Yafen, Li, Dayong, Zhang, Huijuan, Hong, Yongbo, Huang, Lei, Liu, Shixia, Li, Xiaohui, Ouyang, Zhigang, Song, Fengming“…RESULTS: The tomato SlHUB1 and SlHUB2 had H2B monoubiquitination E3 ligases activity in vitro and expression of SlHUB1 and SlHUB2 was induced by infection of B. cinerea and Pst DC3000 and by treatment with salicylic acid (SA) and 1-amino cyclopropane-1-carboxylic acid (ACC). Silencing of either SlHUB1 or SlHUB2 in tomato plants showed increased susceptibility to B. cinerea, whereas silencing of SlHUB1 resulted in increased resistance against Pst DC3000. …”
Publicado 2015
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112000por Wu, Jing, Mu, Lixian, Zhuang, Li, Han, Yi, Liu, Tong, Li, Jun, Yang, Yuan, Yang, Hailong, Wei, Lin“…Quantitative PCR (qPCR) was performed to analyze the mRNA expression of the inducible NO synthase (iNOS) and pro-inflammatory cytokines. Nitric oxide (NO) generation was examined using a Griess assay and the secretion of pro-inflammatory cytokines was determined by an enzyme-linked immunosorbent assay (ELISA). …”
Publicado 2015
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