“…For high-affinity binding of wild-type IL-4 to its receptor IL-4Rα, only two of these clusters (i.e. cluster 1 centered around Glu9 and cluster 2 around Arg88) contribute significantly to the free binding energy. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two stable wild-type TP53 5-FU-resistant cell lines, ContinB and ContinD, generated from the HCT116 colon cancer cell line, demonstrate moderate and strong resistance to 5-FU, respectively, markedly-reduced levels of 5-FU-induced apoptosis, and alterations in expression levels of a number of key cell cycle- and apoptosis-regulatory genes as a result of resistance development. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: Initial experiment described in the wild and captive conditions the gonad development and molt (only in captives) cycles over a 12-month period. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: An expression analysis was performed using urinary bladders isolated from sensitized wild type (WT) and NK(1)R(-/- )mice that were stimulated with saline, LPS, or antigen to provoke inflammation. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins), composed of Gα, Gβ, and Gγ subunits, are positioned at the inner face of the plasma membrane and relay signals from activated G protein-coupled cell surface receptors to various signaling pathways. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…AMD473 ICLs in the CH1 HOC cell line were slowly formed and showed no visible signs of being repaired 24 h after removal of drug. …”
Enlace del recurso
Enlace del recurso

“…Expression of SkEIIIK in dysgenic myotubes restored both evoked contractions and depolarization-dependent intracellular Ca(2+) transients with parameters of voltage dependence (V(0.5) = 6.5 ± 3.2 mV and k = 9.3 ± 0.7 mV, n = 5) similar to those for the wild-type DHPR (Garcia, J., T. Tanabe, and K.G. Beam. 1994. …”
Enlace del recurso
Enlace del recurso

“…The only construct that caused a dominant phenotype in wild-type embryos, was Epb4.1l5(long+short_PBD), which caused brain ventricle defects and edema that were similar to those observed in moe(- )mutants. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we report time-related changes and cell line specific patterns of gene expression after CPT treatment by using two p53 wild-type glioblastoma cell lines, U87-MG and DBTRG-05, with different sensitivities to TopoI inhibition. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Experiments in-vitro utilized cultured epidermal keratinocytes generated from wild-type or from P2X(7)-null mice. Assays involved protein immunostaining and Western blots; mRNA real-time qPCR; and apoptosis (evaluated in situ by TUNEL and quantified in cultured keratinocytes as solubilized DNA or by ELISA). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Results: In wild type mice, MI/R significantly decrease myocardial ejection fraction and increased serum troponin I levels and myocardial PMN infiltration compared to sham-operated animals. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Although hydrolysis probe analysis of kdr is still held as the gold standard assay, this paper highlights the importance of kdr mutation confirmation via sequencing especially in regions where kdr frequency has never been reported before or where both the kdr-e and kdr-w mutations are present simultaneously.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Since there are considerable similarities in the GABAergic system in the brain and pancreas, we evaluated the protective role of taurine in pancreatic islet development in both wild type (WT) and fragile X mice (KO). METHODS: One-month-old FVB/NJ males or age-matched fmr1-knockout (KO) mice were supplemented with taurine in drinking water (0.05% w/v) for four weeks. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The K(M)-value was only changed in variants mutated at position 221 (N221S) and was in all cases monitored as a 2-3 × increase for pNPGlc, while the K(M )decreased a corresponding extent for Q3. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: In two genetic backgrounds (129/Bl-6 and FVB) the neo-natal RARα1/KO-mammary epithelial tree was two-fold larger and the pubertal tree had two-fold more branch points and five-fold more mature end buds, a phenotype that was predominantly epithelial cell autonomous. The stem/progenitor compartment of the RARα1/KO mammary, defined as CD24(low)/ALDH(high activity )was increased by a median 1.7-fold, but the mammary stem cell (MaSC)-containing compartment, (CD24(low)/CD29(high)), was larger (approximately 1.5-fold) in the wild type (wt)-glands, and the mammary repopulating ability of the wt-gland epithelium was approximately two-fold greater. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: Mutations in the K-RAS gene (mutRAS) were detected in 23 patients (32%) with the remaining 48 patients (68%) having wild-type RAS (wtRAS). The percent of blast cells was significantly lower in mutRAS compared to wtRAS patients (P ≤ 0.001) while M4 subtype of AML and Inv(16) frequencies were significantly higher in mutRAS compared to wtRAS patients (P = 0.015) and (P = 0.003), respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…There, several tuber species were domesticated and the area lodges one of the most important reservoirs of their varieties and wild relatives. It is also the setting of traditional cultures using and conserving them. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The addition of EGFR siRNA to either TKIs or cetuximab additively enhanced growth inhibition and induction of apoptosis in all five cell lines, independent of the EGFR mutation status (wild-type or sensitizing mutation or resistant mutation). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…METHODS: Global gene expression patterns were measured in T47D and MCF-7 breast cancer cells expressing either wild-type (SUMOylation-capable) or K388R (SUMOylation-deficient) PRs and subjected to pathway analysis. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Preoperative (neoadjuvant) chemotherapy and radiotherapy are more effective than similar postoperative treatment for oesophageal, gastric, and rectal cancers, perhaps because of more effective micrometastasis eradication and reduced risk of incomplete excision and tumour cell shedding during surgery. The FOxTROT trial aims to investigate the feasibility, safety, and efficacy of preoperative chemotherapy for colon cancer. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso