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4741por Wang, Youjin, Savage, Sharon A., Alsaggaf, Rotana, Aubert, Geraldine, Dagnall, Casey L., Spellman, Stephen R., Lee, Stephanie J., Hicks, Belynda, Jones, Kristine, Katki, Hormuzd A., Gadalla, Shahinaz M.“…This study aimed to assess the validity of converting the quantitative polymerase chain reaction (qPCR) telomere/single copy gene (T/S) ratio to TL in kilobases (kb). We developed a linear regression equation to predict TL from qPCR T/S using flow cytometry with fluorescence in situ hybridization (flow FISH) TL data from 181 healthy donors (age range = 19–53) from the National Marrow Donor Program (NMDP) biorepository. …”
Publicado 2018
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4742por Tsatsakis, Aristidis, Tsoukalas, Dimitrios, Fragkiadaki, Persefoni, Vakonaki, Elena, Tzatzarakis, Manolis, Sarandi, Evangelia, Nikitovic, Dragana, Tsilimidos, Gerasimos, Alegakis, Athanasios K.“…Basic statistical analyses of TL data were provided through BIOTEL, including TL percentiles, specialized charts for TL distribution including the percentage of critically short telomeres (< 3,000 kilobases), individual telomere profiles, and graphs of biological age vs. chronological age. …”
Publicado 2019
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4743por Joaquim, Letícia Borges, Chud, Tatiane Cristina Seleguim, Marchesi, Jorge Augusto Petroli, Savegnago, Rodrigo Pelicioni, Buzanskas, Marcos Eli, Zanella, Ricardo, Cantão, Mauricio Egidio, Peixoto, Jane Oliveira, Ledur, Mônica Correa, Irgang, Renato, Munari, Danísio Prado“…The average linkage disequilibrium (r(2)) was 0.291 ± 0.312 for all adjacent SNPs, distancing less than 100 Kb (kilobase) between markers. The average inbreeding coefficients obtained from runs of homozygosity (ROH) and pedigree analyses were 0.119 and 0.0001, respectively. …”
Publicado 2019
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4744“…The study focused on wastes resulting from the wet gravitation and flotation processes employed for the purposes of coking coal cleaning in four coal mines situated in the Upper Silesian Coal Basin (Poland). …”
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4745por Howard, Alicia D., Wang, Xiaochun, Prasad, Megana, Sahu, Avinash Das, Aniba, Radhouane, Miller, Michael, Hannenhalli, Sridhar, Chang, Yen-Pei Christy“…Our results demonstrate that through our approach, the LD blocks that contain GWAS signals, often hundreds of kilobases in size with multiple SNPs serving as statistical proxies to the true functional site, can provide an experimentally testable hypothesis for the underlying regulatory mechanism linking genetic variants to complex traits.…”
Publicado 2019
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4746por Tangtrongsup, Sahatchai, Sripakdee, Duanghatai, Malaivijitnond, Suchinda, Angkuratipakorn, Rungroj, Lappin, Michael“…Intestinal parasitic infections were examined by zinc sulfate centrifugation flotation and by a commercially available immunofluorescent assay (IFA) for detection of G. duodenalis and Cryptosporidium spp.. …”
Publicado 2019
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4747“…Read length N50 ranged between 5.3 and 5.4 kilobase pairs over the 4 sequencing runs. Basecalls and corresponding signal data are made available (4.2 TB in total). …”
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4748por Boyer, Audrey, Dreneau, Julie, Dumans, Amélie, Burlaud-Gaillard, Julien, Bull-Maurer, Anne, Roingeard, Philippe, Meunier, Jean-Christophe“…When this lysis buffer was used on HCV-infected cells and the resulting lysates were subjected to flotation gradient centrifugation, all viral proteins and DRM-resident proteins were found in soluble protein fractions. …”
Publicado 2019
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4749por Chae, Jeong-Byoung, Park, Jinho, Jung, Suk-Han, Kang, Jin-Hee, Chae, Joon-Seok, Choi, Kyoung-Seong“…No Eimeria oocysts were detected using the flotation method. In comparison with post-weaned calves with diarrhea, in diarrhea-recovered calves, the lymphocyte count was significantly higher (P = 0.018), and the monocyte count was significantly lower (P = 0.001); however, the number of monocytes was still high. …”
Publicado 2019
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4750por Husain, Mureed, Tufail, Muhammad, Mehmood, Khalid, Rasool, Khawaja Ghulam, Aldawood, Abdulrahman Saad“…Among the six Vg gene transcripts, only one was highly expressed with 3234.95 FPKM value (fragments per kilobase per million mapped reads) that was much higher than that of the other five transcripts. …”
Publicado 2019
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4751por Eisen, Ana Karolina Antunes, Demoliner, Meriane, de Oliveira, Kelen Gras, Troian, Eduardo Artur, Mallmann, Larissa, Filippi, Micheli, de Almeida, Paula Rodrigues, Spilki, Fernando Rosado“…Parasitological analyzes were conducted through flotation and sedimentation techniques, and polymerase chain reaction (PCR) was used for viral detection. …”
Publicado 2019
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4752“…Total number of born piglets per litter and parity of the sow were recorded and stillborn piglets were identified using a lung flotation technique. The association between HbC in sows and piglets was determined using a linear mixed model. …”
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4753por Feng, Ye, Chang, Yi-Jung, Fang, Szu-Hsuan, Su, Lin-Hui, Li, Hsin-Chieh, Yang, Hsin-Ping, Yu, Min-Jia, Chiu, Cheng-Hsun“…The MIC(90) of ceftriaxone and ciprofloxacin for the outbreak isolates was >256 μg/mL and 1 μg/mL, respectively, and a conjugative 278-kilobase plasmid harboring bla(CTX-M-55) and qnrS1 contributed towards the resistance. …”
Publicado 2019
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4754por Morton, Elizabeth A., Hall, Ashley N., Kwan, Elizabeth, Mok, Calvin, Queitsch, Konstantin, Nandakumar, Vivek, Stamatoyannopoulos, John, Brewer, Bonita J., Waterston, Robert, Queitsch, Christine“…Repeat units of rDNA are kilobases in size, often with several hundred units comprising the array, making rDNA particularly intractable to common quantification techniques. …”
Publicado 2019
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4755“…The flag-2 loci range in size from ~ 3.4 to 81.1 kilobases and code for between five and 102 distinct proteins. …”
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4756por Miller, Danny E.“…The data indicate that, in the absence of SC, copy-number variation still occurs and meiotic DSB repair by gene conversion occurs infrequently. Remarkably, an 856-kilobase de novo CNV was observed in two unrelated individuals of different genetic backgrounds and was identical to a CNV recovered in a previous wild-type study, suggesting that recurrent formation of large CNVs occurs in Drosophila. …”
Publicado 2019
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4757“…Coronavirus genomes (~ 26–32 kilobases) are the largest RNA genomes known to date and their expansion was likely enabled by acquiring enzyme functions that counter the commonly high error frequency of viral RNA polymerases. …”
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4758“…Coronaviruses (26–34 kilobases) have the largest known RNA genomes and their replication presumably requires a processive RNA-dependent RNA polymerase (RdRp) and enzymatic functions that suppress the consequences of the typically high error rate of viral RdRps. …”
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4759“…A dot blot hybridization assay was developed for use as a rapid screening test to detect bovine viral diarrhea virus (BVDV) in serum from infected cattle. A 1.1. kilobase cDNA, prepared from the BVDV genome, was molecularly cloned and used in this study. …”
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4760por Perrucci, Stefania, Berrilli, Federica, Procopio, Cristina, Di Filippo, Margherita Montalbano, Pierini, Alessio, Marchetti, Veronica“…For parasitological analysis, fresh fecal samples collected from each dog were examined by fresh and Lugol stained smears, flotation test, and a rapid immunoassay. Giardia duodenalis genotypes were identified by molecular analysis. …”
Publicado 2020
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