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4821“…METHODOLOGY/PRINCIPAL FINDINGS: Nucleotide sequences from 32 unlinked loci (>26 Kilobases of aligned sequence) were used to reconstruct the evolutionary history of strains representing the known genetic variability of T. cruzi. …”
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4822“…RESULTS: In this paper, by using five published RNA-Seq data sets from different biological sources and with different data preprocessing schemes, we showed that commonly used estimates of gene expression levels from RNA-Seq data, such as reads per kilobase of gene length per million reads (RPKM), are biased in terms of gene length, GC content and dinucleotide frequencies. …”
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4823“…Genomic DNA analysis identified a 1.5 kilobase deletion, spanning exon 3 and including extended flanking intronic sequence. …”
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4824por Zhang, Weizhe, Shen, Yujuan, Wang, Rongjun, Liu, Aiqin, Ling, Hong, Li, Yihong, Cao, Jianping, Zhang, Xiaoyun, Shu, Jing, Zhang, Longxian“…Cryptosporidium oocysts and Giardia cysts were detected using Sheather's sugar flotation technique and Lugol's iodine stain method, respectively. …”
Publicado 2012
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4825“…We develop a statistical mechanical model to analyze the competitive behavior of transitions to multiple alternate conformations in a negatively supercoiled DNA molecule of kilobase length and specified base sequence. Since DNA superhelicity topologically couples together the transition behaviors of all base pairs, a unified model is required to analyze all the transitions to which the DNA sequence is susceptible. …”
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4826“…We characterized the products of 5,860 female meioses in Drosophila melanogaster by genotyping a total of 139 million informative SNPs and mapped 106,964 recombination events at a resolution down to 2 kilobases. This approach allowed us to generate whole-genome CO and GC maps as well as a detailed description of variation in recombination among individuals of this species. …”
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4827por Cuervo, Naomi, Fernandez, Barbara, Rodriguez, Jesus, Murillo, Celio, Perez, Tila M., Juarez, Daniel“…The mites were isolated by the sedimentation flotation method Spieskma-Boezeman 1967. All the identified material was deposited in a Basic Collection from Rocel Laboratories in Puebla and in the National Collection of Acarology from the Institute of Biology, UNAM, Mexico. …”
Publicado 2012
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4828por Flegel, Caroline, Manteniotis, Stavros, Osthold, Sandra, Hatt, Hanns, Gisselmann, Günter“…In total, 111 of 400 OR genes were expressed with an FPKM (fragments per kilobase of exon per million fragments mapped) higher than 0.1 in at least one tissue. …”
Publicado 2013
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4829“…We introduce a novel simulation of a 100-kilobase gene region, based on the standard definition of a gene, in which mutations are unconditionally deleterious, are continuously arising, have partially recessive and non-complementing effects on phenotype (analogous to what is widely observed for most Mendelian disorders), and are interspersed with neutral markers that can be genotyped. …”
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4830por Capra, John A., Hubisz, Melissa J., Kostka, Dennis, Pollard, Katherine S., Siepel, Adam“…We implemented our model in a computer program, called phastBias, that can accurately detect gBGC tracts about 1 kilobase or longer in simulated sequence alignments. …”
Publicado 2013
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4831por Bobrie, Angélique, Colombo, Marina, Krumeich, Sophie, Raposo, Graça, Théry, Clotilde“…By electron microscopy, CD9 was observed on vesicles of various sizes, ranging from 30 nm to more than 150 nm in diameter. Flotation onto sucrose gradients showed different proportions of CD63, CD9 and Mfge8 not only in fractions of densities classically described for exosomes (around 1.15 g/ml) but also in fractions of densities over 1.20 g/ml, indicating the presence of heterogenous vesicle populations. …”
Publicado 2012
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4832por Kalra, Shikha, Puniya, Bhanwar Lal, Kulshreshtha, Deepika, Kumar, Sunil, Kaur, Jagdeep, Ramachandran, Srinivasan, Singh, Kashmir“…Large scale expression profiling through Reads per Kilobase per Million mapped reads (RPKM) showed major genes involved in different metabolic pathways of the plant. …”
Publicado 2013
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4833“…The synthesis of the negative-strand [(−)-strand] complement of the ∼30 kilobase, positive-strand [(+)-strand] coronaviral genome is a necessary early step for genome replication. …”
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4834por Bortolamiol, Sarah, Cohen, Marianne, Potts, Kevin, Pennec, Flora, Rwaburindore, Protase, Kasenene, John, Seguya, Andrew, Vignaud, Quentin, Krief, SabrinaEnlace del recurso
Publicado 2014
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4835“…We find that Gro predominantly binds at discrete peaks (<1 kilobase). We also demonstrate that blocking Gro oligomerization does not reduce peak width as would be expected if Gro oligomerization induced spreading along the chromatin from the site of recruitment. …”
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4836por Cornelissen, Jan B. W. J., van der Giessen, Joke W. B., Takumi, Katsuhisa, Teunis, Peter F. M., Wisselink, Henk J.“…Oocysts shed by these five groups of cats were collected from faeces by flotation techniques, counted microscopically and estimated by real time PCR. …”
Publicado 2014
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4837“…To handle such information sharing in a rigorous manner, we propose an hierarchical, empirical Bayes approach (R-EBSeq) that combines the Cufflinks model for generating relative transcript abundance measurements, known as FPKM (fragments per kilobase of transcript length per million mapped reads) with the EBArrays framework, which was previously developed for empirical Bayes analysis of microarray data. …”
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4838“…Based on measures of FPKG (fragments mapped per kilobase of gene length), genes involved in methanogenesis, in addition to a spirochaete flagellin gene, were among those most highly expressed in Wg25. …”
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4839por Legault, Marc-André, Girard, Simon, Lemieux Perreault, Louis-Philippe, Rouleau, Guy A., Dubé, Marie-Pierre“…CNVs are genomic structural variations defined as a variation in the number of copies of a large genomic fragment, usually more than one kilobase. Here, we aim to compare different CNV calling methods in order to assess their ability to consistently identify CNVs by comparison of the calls in 9 quartets of identical twin pairs. …”
Publicado 2015
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4840“…For chicken, PCR amplifications of fragments of about 1.5 kilobases are highly likely to represent true mitochondrial amplification. …”
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