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4941por Yoshiji, Satoshi, Iwasaki, Yorihiro, Iwasaki, Kanako, Honjo, Sachiko, Hirano, Koichi, Ono, Katsuhiko, Yamazaki, Yuto, Sasano, Hironobu, Hamasaki, Akihiro“…Subsequent quantitative polymerase chain reaction (qPCR)–based copy number mapping showed a monoallelic loss of approximately 18.5-kilobase region containing the whole MEN1 gene. Intriguingly, the 2 breakpoints were flanked by Alu repetitive elements, suggesting the contribution of Alu/Alu-mediated rearrangements (AAMR) to the whole MEN1 gene deletion. …”
Publicado 2020
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4942por Archambeault, Sophie L., Bärtschi, Luis R., Merminod, Aurélie D., Peichel, Catherine L.“…Freshwater ecotypes have repeatedly fixed a 16 kilobase haplotype on chromosome IV that contains Ectodysplasin (Eda), a gene known to affect multiple traits, including defensive armor plates, lateral line sensory hair cells, and schooling behavior. …”
Publicado 2020
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4943por Tiika, Richard John, Wei, Jia, Ma, Rui, Yang, Hongshan, Cui, Guangxin, Duan, Huirong, Ma, Yanjun“…Moreover, the expression levels of 23 selected genes with fragments per kilobase of exons per million mapped reads (FPKM) >5 were assayed during different fruit developmental stages with real-time quantitative polymerase chain reaction (RT-qPCR). …”
Publicado 2020
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4944“…Coronavirus have the largest RNA genomes (~26–32 kilobases) and their expansion was likely enabled by acquiring enzyme functions that counter the commonly high error frequency of viral RNA polymerases. …”
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4945por Bahrami, Ataallah, Abdollahi, Morteza, Mirmohammadi, Mirsaleh, Kazemi, Fatemeh, Danesh, Abolfazl, Shokrzadeh, Maryam“…It can even be concluded that the mill has a destructive effect the flotation process by producing slimes.…”
Publicado 2020
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4946por Ruth, Matsinkou Mba Rosine, Cedric, Yamssi, Malla, Mbong Erica, Nadia, Noumedem Anangmo Christelle, Aime, Tateng Ngouateu, Leonelle, Megwi, Payne, Vincent Khan“…Qualitative and quantitative analyses of stool samples were done using the simple flotation and McMaster count techniques, respectively. …”
Publicado 2021
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4947por Xu, Shanghua, Zhang, Shumiao, Hu, Xiaolong, Zhang, Baofeng, Yang, Shuang, Hu, Xin, Liu, Shuqiang, Hu, Defu, Bai, Jiade“…METHODS: In total, 1,345 Père David’s deer faecal samples from four regions during four seasons were tested using the flotation (saturated sodium nitrate solution) to identify parasites of different genus or group, and the McMaster technique to count the number of eggs or oocysts. …”
Publicado 2021
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4948“…Differential gene expression analysis, differential Gene Ontology (GO) enrichment analysis and differential Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out, and the reads per kilobase per million reads (RPKM) value was used as a measure of gene expression. …”
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4949por Yilmaz, Feyza, Null, Megan, Astling, David, Yu, Hung-Chun, Cole, Joanne, Santorico, Stephanie A., Hallgrimsson, Benedikt, Manyama, Mange, Spritz, Richard A., Hendricks, Audrey E., Shaikh, Tamim H.“…RESULTS: We identified over 400,000 CNVs larger than 1 kilobase (kb), for an average of 120 CNVs (SE = 2.57) per individual. …”
Publicado 2021
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4950“…Some jumbo phages, those with a genome size larger than 200 kilobases, have recently been discovered to establish complex subcellular organization during replication. …”
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4951por Tvedte, Eric S, Gasser, Mark, Sparklin, Benjamin C, Michalski, Jane, Hjelmen, Carl E, Johnston, J Spencer, Zhao, Xuechu, Bromley, Robin, Tallon, Luke J, Sadzewicz, Lisa, Rasko, David A, Dunning Hotopp, Julie C“…The newest generation of DNA sequencing technology is highlighted by the ability to generate sequence reads hundreds of kilobases in length. Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) have pioneered competitive long read platforms, with more recent work focused on improving sequencing throughput and per-base accuracy. …”
Publicado 2021
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4952por Song, Miaoyu, Wang, Haomiao, Wang, Zhe, Huang, Hantang, Chen, Shangwu, Ma, Huiqin“…Seventy-two bHLHs were found expressed at fragments per kilobase per million mapped (FPKM) > 10 in the fig fruit; among them, 15 bHLHs from eight subfamilies had FPKM > 100 in at least one sample. bHLH subfamilies had different expression patterns in the female flower tissue and peel during fig fruit development. …”
Publicado 2021
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4953“…Repeat motif size can be a single nucleotide to kilobases/unit. At a given locus, repeat motif sequence purity can vary with consequence. …”
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4954por Kim, Jong-Myoung, Kim, Youn-Sook, Kim, Yeo-Reum, Choi, Mi-Jin, DasSarma, Priya, DasSarma, Shiladitya“…ABSTRACT: Gas vesicle nanoparticles (GVNPs) are hollow, buoyant prokaryotic organelles used for cell flotation. GVNPs are encoded by a large gas vesicle protein (gvp) gene cluster in the haloarchaeon, Halobacterium sp. …”
Publicado 2022
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4955por Nath, Tilak Chandra, Eom, Keeseon S., Choe, Seongjun, Islam, Saiful, Sabuj, Siblee Sadik, Saha, Eva, Tuhin, Rumman Hossain, Ndosi, Barakaeli Abdieli, Kang, Yeseul, Kim, Sunmin, Bia, Mohammed Mebarek, Park, Hansol, Lee, Dongmin“…Parasitological assessment was done using the combined sedimentation-flotation method, and factors associated with infection were modeled using mixed-effects logistic regression. …”
Publicado 2022
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4956por Mohd-Qawiem, Firdaus, Nur-Fazila, Saulol Hamid, Ain-Fatin, Raslan, Yong, Qian Hui, Nur-Mahiza, Md Isa, Yasmin, Abd Rahaman“…Various parasitological techniques such as perianal tape test, simple flotation, direct examination of the intestine, and fecal smear were performed for intestinal parasite detection; hair plucking, skin scraping, and full body combing for ectoparasite identification; and blood smear, microhematocrit centrifugation, and buffy coat techniques for blood parasite detection. …”
Publicado 2022
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4957“…To detect oocysts of Cryptosporidium spp. in feces, the flotation method was used, and slides were stained using the modified Ziehl-Neelsen method. …”
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4958por Yamauchi, Kozue, Sato, Mitsuaki, Osawa, Leona, Matsuda, Shuya, Komiyama, Yasuyuki, Nakakuki, Natsuko, Takada, Hitomi, Katoh, Ryo, Muraoka, Masaru, Suzuki, Yuichiro, Tatsumi, Akihisa, Miura, Mika, Takano, Shinichi, Amemiya, Fumitake, Fukasawa, Mitsuharu, Nakayama, Yasuhiro, Yamaguchi, Tatsuya, Inoue, Taisuke, Maekawa, Shinya, Enomoto, Nobuyuki“…RCA of the circularized reverse‐transcription polymerase chain reaction products successfully produced DNA longer than 30 kilobase pairs (kb) containing multiple tandem repeats of a target 3 kb HCV genome. …”
Publicado 2022
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4959por Mossion, Vinciane, Dauphin, Benjamin, Grant, Jason, Kessler, Michael, Zemp, Niklaus, Croll, Daniel“…Based on read mapping to reference transcript sequences, we identified 374,463 single nucleotide polymorphisms (SNPs) segregating among individuals for an average density of 14 SNPs per kilobase. We found that all 12 transcriptomes were most likely from diploid individuals. …”
Publicado 2021
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4960“…The samples were pretreated by drying, flotation separation using NaCl solution, and digestion by H(2)O(2). …”
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