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5001por Lynsdale, Carly L., Santos, Diogo J. Franco dos, Hayward, Adam D., Mar, Khyne U., Htut, Win, Aung, Htoo Htoo, Soe, Aung Thura, Lummaa, Virpi“…The quantitative assessment of parasite infection is necessary to measure, manage and reduce infection risk in both wild and captive animal populations. Traditional faecal flotation methods which aim to quantify parasite burden, such as the McMaster egg counting technique, are widely used in veterinary medicine, agricultural management and wildlife parasitology. …”
Publicado 2015
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5002Genome-wide annotation of microRNA primary transcript structures reveals novel regulatory mechanismspor Chang, Tsung-Cheng, Pertea, Mihaela, Lee, Sungyul, Salzberg, Steven L., Mendell, Joshua T.“…While a subset of miRNAs are hosted in protein-coding genes, the majority of pri-miRNAs are transcribed as poorly characterized noncoding RNAs that are 10's to 100's of kilobases in length and low in abundance due to efficient processing by the endoribonuclease DROSHA, which initiates miRNA biogenesis. …”
Publicado 2015
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5003“…In recent years, RNA-seq is emerging as a powerful technology in estimation of gene and/or transcript expression, and RPKM (Reads Per Kilobase per Million reads) is widely used to represent the relative abundance of mRNAs for a gene. …”
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5004“…We employ a molecular taxonomic dataset using ~2 kilobases nuDNA (Wg, LW Rh, Abd-A, 28s) and 790 basepairs mtDNA (CO1), along with geographic and habitat data, to examine the diversification patterns of the ant genus Mystrium Roger, 1862, (Subfamily Amblyoponinae) from Madagascar. …”
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5005por Irigoyen, Nerea, Firth, Andrew E., Jones, Joshua D., Chung, Betty Y.-W., Siddell, Stuart G., Brierley, Ian“…Members of the family Coronaviridae have the largest genomes of all RNA viruses, typically in the region of 30 kilobases. Several coronaviruses, such as Severe acute respiratory syndrome-related coronavirus (SARS-CoV) and Middle East respiratory syndrome-related coronavirus (MERS-CoV), are of medical importance, with high mortality rates and, in the case of SARS-CoV, significant pandemic potential. …”
Publicado 2016
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5006“…FEC data were analysed from 2175 cattle on 52 farms using a McMaster method at two different diagnostic sensitivities (30 and 15 eggs per gram (epg)) and a sensitive centrifugal flotation technique (SCFT) with a sensitivity of 1 epg. …”
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5007por Collino, Federica, Pomatto, Margherita, Bruno, Stefania, Lindoso, Rafael Soares, Tapparo, Marta, Sicheng, Wen, Quesenberry, Peter, Camussi, Giovanni“…The distribution of exosomal markers and miRNAs evaluated in the twelve gradient fractions showed an enrichment in fractions with a flotation density of 1.08–1.14 g/mL. Based on this observation, we evaluated the biological activity on renal cell proliferation and apoptosis resistance of low (CF1), medium (CF2) and high (CF3) floatation density fractions. …”
Publicado 2017
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5008“…We illustrate the premise with the data on recombinant mt genome of the Siberian taimen Hucho taimen (NCBI Reference Sequence Database NC_016426.1; GenBank accession number HQ897271.1), bearing 2 introgressed fragments (≈0.9 kb [kilobase]) from 2 lenok subspecies, Brachymystax lenok and Brachymystax lenok tsinlingensis, submitted to GenBank on June 12, 2011. …”
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5009por Mayer, Manfred, Unterseer, Sandra, Bauer, Eva, de Leon, Natalia, Ordas, Bernardo, Schön, Chris-Carolin“…When combining data of up to 10 landraces, LD decay distances decreased to a few kilobases. Genotyping 24 individuals per landrace with 5k SNPs was sufficient for obtaining representative estimates of diversity and LD levels to allow an informed pre-selection of landraces. …”
Publicado 2017
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5010por Ross, James L., Cooper, Lee A. D., Kong, Jun, Gutman, David, Williams, Merete, Tucker-Burden, Carol, McCrary, Myles R., Bouras, Alexandros, Kaluzova, Milota, Dunn, William D., Duong, Duc, Hadjipanayis, Constantinos G., Brat, Daniel J.Enlace del recurso
Publicado 2017
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5011por Baca, Katharyn M., Govil, Manika, Zmuda, Joseph M., Simhan, Hyagriv N., Marazita, Mary L., Bodnar, Lisa M.“…Single‐nucleotide polymorphisms (SNPs) were genotyped 50 kilobases up‐ and down‐stream in three genes (VDR,GC, and CYP27B1) in the samples from both studies, for a total of 744 preeclampsia cases and 2411 controls. …”
Publicado 2018
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5012por Takata, Matthew A., Soll, Steven J., Emery, Ann, Blanco-Melo, Daniel, Swanstrom, Ronald, Bieniasz, Paul D.“…The ~9.5 kilobase HIV-1 genome contains RNA sequences and structures that control many aspects of viral replication, including transcription, splicing, nuclear export, translation, packaging and reverse transcription. …”
Publicado 2018
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5013por Kotlan, Beatrix, Plotar, Vanda, Eles, Klara, Horvath, Szabolcs, Balatoni, Timea, Csuka, Orsolya, Újhelyi, Mihaly, Sávolt, Ákos, Szollar, Andras, Vamosi-Nagy, Istvan, Toth, Laszlo, Farkas, Emil, Toth, Jozsef, Kasler, Miklos, Liszkay, GabriellaEnlace del recurso
Publicado 2018
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5014por Klaus, Annette, Zimmermann, Elke, Röper, Kathrin Monika, Radespiel, Ute, Nathan, Senthilvel, Goossens, Benoit, Strube, Christina“…Designed as a follow-up study, prevalence and co-infection status of intestinal parasites from N. larvatus in a protected area in Malaysian Borneo were analyzed from fecal samples using a flotation method. For the first time, the intestinal parasite co-infection patterns were examined using quantitative analyses. …”
Publicado 2017
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5015“…We previously described the identification of a chromosomal deletion in Arabidopsis thaliana that resulted in the elimination of genomic DNA between two T-DNA insertions located ca. 25 kilobases apart on chromosome IV. The mechanism responsible for this deletion appears to have been recombination between the closely spaced T-DNA elements located in trans in a parent plant. …”
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5016“…Of the 15 candidate CADs, four unigenes that possessed high FPKM (fragments per transcript kilobase per million fragments mapped) value in roots were cloned and characterized. …”
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5017“…By examining genome-wide data from over 200,000 markers, including 150 recessive disease variants, we built high-resolution ROH density maps for nearly 2,500 dogs, recording ROH down to 500 kilobases. We observed over 678 homozygous deleterious recessive genotypes in the panel across 29 loci, 90% of which overlapped with ROH inferred by GERMLINE. …”
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5018“…These results thus support DNA charge transport between repair proteins over kilobase distances. The results furthermore highlight the ability of DNA repair proteins to search the genome quickly and efficiently using DNA charge transport chemistry.…”
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5019por Brander, Robert W., Warton, Nicola, Franklin, Richard C., Shaw, Wendy S., Rijksen, Eveline J. T., Daw, Shane“…The majority of bystander rescuers had water safety training (65.8%; n = 160), self-rated as strong swimmers (68.3%; n = 166), conducted the rescue without help from others (60%; n = 146), did not use a flotation device to assist (63%; n = 153), but were confident in their ability to make the rescue (76.5%; n = 186). …”
Publicado 2019
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5020“…Based on the fragments per kilobase million value, differential expression analysis was conducted using the limma package in R. …”
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