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  1. 5121
    por Lin, Hsin-Hung, Liao, Yu-Chieh
    Publicado 2015
    “…Third-generation single-molecule, real-time (SMRT) sequencing technology avoids amplification artifacts and generates kilobase-long reads with the potential to complete microbial genome assembly. …”
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    Online Artículo Texto
    Evaluation and Validation of Assembling Corrected PacBio Long Reads for Microbial Genome Completion via Hybrid Approaches
  2. 5122
    “…CONCLUSIONS: This study is the first of its kind to employ a sucrose flotation gradient assay to map intracellular KSHV trafficking in HFF cells. …”
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    Online Artículo Texto
    Subcellular fractionation method to study endosomal trafficking of Kaposi’s sarcoma-associated herpesvirus
  3. 5123
    “…Further, Fragments Per Kilobase of transcript per Million mapped reads (FPKM) values of the identified secondary metabolite pathway genes were determined and Reverse Transcription PCR (RT-PCR) of a few of these genes were performed to validate the de novo assembled leaf transcriptome dataset. …”
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    Sequencing, De novo Assembly, Functional Annotation and Analysis of Phyllanthus amarus Leaf Transcriptome Using the Illumina Platform
  4. 5124
    “…The methylation status of the MDR1 gene promoter region 16 CpG units was analyzed by using kilobase-specific cracking and matrix-assisted laser desorption ionization time of flight mass spectrometry. …”
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    Online Artículo Texto
    Influence of MDR1 methylation on the curative effect of interventional embolism chemotherapy for cervical cancer
  5. 5125
    “…CNV was bioinformatically predicted and affected ~300–400 Kilobases (Kb) of the C. gattii VGII subgroup genomes. …”
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    Online Artículo Texto
    Copy number variation contributes to cryptic genetic variation in outbreak lineages of Cryptococcus gattii from the North American Pacific Northwest
  6. 5126
    “…Using the combined sedimentation-flotation method, 61.4 % (58.2–64.5 %, 95 % confidence interval [CI]) tested positive for one or more gastrointestinal helminths, namely, strongyles (57.1 %, 95 % CI), Metastrongylus spp. (7.6 %, 95 % CI), Ascaris suum (5.9 %, 95 % CI), Strongyloides ransomi (4.2 %, 95 % CI), and Trichuris suis (3.4 %, 95 % CI). …”
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    Online Artículo Texto
    Prevalence and risk factors for gastrointestinal parasites in small-scale pig enterprises in Central and Eastern Uganda
  7. 5127
    “…Stool samples were collected and processed by sedimentation, flotation, Kato-Katz, Baermann-Moraes and Graham methods, iron haematoxylin staining and safranin staining. …”
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    Geospatial distribution of intestinal parasitic infections in Rio de Janeiro (Brazil) and its association with social determinants
  8. 5128
    “…Analysis of (oo)cysts in the feces was performed by the modified Ziehl-Neelsen staining and centrifugal flotation in zinc sulfate. Test-positive samples were subjected to nested PCR amplification of the 18SSU ribosomal RNA gene for identification of Cryptosporidium and Giardia and of the gp60 gene for subtyping of Cryptosporidium. …”
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    Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms
  9. 5129
    “…We found the use of 1% 7X as a surfactant compared to 0.1% Tween 80 significantly improved recovery efficiency (two-sided t-test, t = 5.03, p = 0.007) while other protocol modifications—including different agitation and flotation methods—did not have a significant impact. …”
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    Detecting and enumerating soil-transmitted helminth eggs in soil: New method development and results from field testing in Kenya and Bangladesh
  10. 5130
    “…The rs1619661 variant is on chromosome 10, 132 kilobase (kb) downstream from CXCL12, which encodes a chemokine, stromal cell-derived factor 1, that is expressed in cardiomyocytes and decreases calcium influx across the L-type [Formula: see text] channel. …”
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    Genome-wide Association Study of Susceptibility to Particulate Matter–Associated QT Prolongation
  11. 5131
    “…RNA sequencing (RNA-seq) was performed on a HiSeq 2500 platform and fragments per kilobase of exon per million fragments mapped was utilized to calculate the gene expression value following preprocessing of the RNA-seq data. …”
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    Identification of potentially relevant genes for myocardial infarction using RNA sequencing data analysis
  12. 5132
    “…Using a novel approach based on Reads Per Kilobase per Million Mapped (RPKM), we identified 167 genes predominantly absent in lineage II but present in lineage I. …”
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    Genetic Separation of Listeria monocytogenes Causing Central Nervous System Infections in Animals
  13. 5133
    “…An RNA-seq approach showed markedly different hemocyanin mRNA levels from ~ 6 to 25,000 reads per kilobase per million reads. To evaluate the contribution of hemocyanin to O(2) transport, we specifically studied the hemocyanin of the centipede Scolopendra dehaani. …”
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    Diversity, evolution, and function of myriapod hemocyanins
  14. 5134
    “…Ov.RS3 exhibited 10 kilobase to 10 megabase deletions and tandem duplications, and patients whose tumors exhibited a high contribution from Ov.RS3 had poor OS. …”
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    Genomic Rearrangement Signatures and Clinical Outcomes in High-Grade Serous Ovarian Cancer
  15. 5135
    “…METHODOLOGY AND PRINCIPLE FINDINGS: To investigate the occurrence of genetic variation in Cs. melanura, the genome of this mosquito vector was sequenced resulting in a draft genome assembly of 1.28 gigabases with a contig N50 of 93.36 kilobases. Populations of Cs. melanura from 10 EEE virus foci in the eastern North America were genotyped with double-digest RAD-seq. …”
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    Population genomics of Culiseta melanura, the principal vector of Eastern equine encephalitis virus in the United States
  16. 5136
    “…RNA viruses are the only known RNA-protein (RNP) entities capable of autonomous replication (albeit within a permissive host environment). A 33.5 kilobase (kb) nidovirus has been considered close to the upper size limit for such entities; conversely, the minimal cellular DNA genome is in the 100–300 kb range. …”
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    A planarian nidovirus expands the limits of RNA genome size
  17. 5137
    “…The isolation of endoparasite eggs/cysts from the fecal samples was by the centrifugation flotation and sedimentation method. All the sampled groups, except one, had an infection of at least one endoparasite taxa, and a total of 21 endoparasite taxon were recorded. …”
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    Prevalence of gastrointestinal parasites in bonnet macaque and possible consequences of their unmanaged relocations
  18. 5138
    “…Optical maps for four randomly selected T-DNA lines revealed between one and seven insertions/rearrangements, and the length of individual insertions from 27 to 236 kilobases. De novo nanopore sequencing-based assemblies for two segregating lines partially resolved T-DNA structures and revealed multiple translocations and exchange of chromosome arm ends. …”
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    The complex architecture and epigenomic impact of plant T-DNA insertions
  19. 5139
    “…METHODS: We used both coprological methods (flotation and sedimentation; N = 1,138 fecal samples) and molecular identification techniques (rDNA and mtDNA; N = 18 larvae) to identify gastrointestinal parasites in nine sympatric host species (cattle, sheep, goats, wildebeest, Grant’s gazelles, Thomson’s gazelles, impala, vervet monkeys and baboons) in the Amboseli ecosystem, Kenya. …”
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    Infection dynamics of gastrointestinal helminths in sympatric non-human primates, livestock and wild ruminants in Kenya
  20. 5140
    “…Moreover, the accumulation of PAF102 in the yeast lipid droplets facilitates its downstream extraction and recovery by flotation on density gradients, with the recovered PAF102 being biologically active against pathogenic fungi. …”
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    Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris
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