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  1. 5221
    “…The Ensembl transcriptome yielded a total of 1241 unigenes with a total sequence length of 389,126 bp and an average length size of 389 bp (guanine-cytosine content = 49.9%). mRNA abundance was measured by reads per kilobase per million and 41 and 37 ensembl unigenes showed up- and down-regulation respectively. …”
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    Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone
  2. 5222
    “…Gene expression was calculated by the reads per kilobase per million reads measurement, and nor malized with tag count comparison. …”
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    Identification of hub genes and regulatory factors of glioblastoma multiforme subgroups by RNA-seq data analysis
  3. 5223
    “…Parasite eggs were isolated from feces through sieving-flotation, and parasite species were then confirmed using PCR and sequencing. …”
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    Support for targeted sampling of red fox (Vulpes vulpes) feces in Sweden: a method to improve the probability of finding Echinococcus multilocularis
  4. 5224
    “…The newest sequencing technologies, such as Oxford Nanopore Technologies (ONT) can generate kilobases-long reads thus representing a promising tool for TEASV detection in plant and animals. …”
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    Detection of active transposable elements in Arabidopsis thaliana using Oxford Nanopore Sequencing technology
  5. 5225
    “…Existing DMR methods provide lists of Differentially Methylated Regions (DMRs) of up to only few kilobases in length, and cannot check if a target region is differentially methylated. …”
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    Redundancy analysis allows improved detection of methylation changes in large genomic regions
  6. 5226
    “…RESULTS: Circ-FBXW7 is abundantly expressed in the normal human brain (reads per kilobase per million mapped reads [RPKM] = 9.31). The spanning junction open reading frame in circ-FBXW7 driven by internal ribosome entry site encodes a novel 21-kDa protein, which we termed FBXW7-185aa. …”
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    Novel Role of FBXW7 Circular RNA in Repressing Glioma Tumorigenesis
  7. 5227
    “…The results showed that the plasmids harboring the bla(NDM−1) gene ranged in size from approximately 52–58 kilobases. Our study indicates that carbapenem-resistant E. cloacae strains that produce NDM carbapenemase have strong resistance. …”
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    Molecular Characterization of Carbapenem-Resistant Enterobacter cloacae in 11 Chinese Cities
  8. 5228
    “…The rapid development in the last 10-15 years of microarray technologies, such as oligonucleotide array Comparative Genomic Hybridization (CGH) and Single Nucleotide Polymorphisms (SNP) genotyping array, has improved the identification of fine chromosomal structural variants, ranging in length from kilobases (kb) to megabases (Mb), as an important cause of genetic differences among healthy individuals and also as disease-susceptibility and/or disease-causing factors. …”
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    A Review of Copy Number Variants in Inherited Neuropathies
  9. 5229
    por So, R T Y, Oladipo, J O, Chu, D K W, Peiris, M
    Publicado 2019
    “…Coronaviruses (CoVs) are enveloped, single stranded, positive-sense RNA viruses with a large genomic size of 26–32 kilobases. The first human CoV identified in the 1960s was isolated from patients presenting with common cold symptoms. …”
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    A54 Genomic analysis of camel-HKU23 in Nigeria dromedary camels reveals strain-specific cross-species recombination
  10. 5230
    “…It automatically computes the reads per kilobase per million reads mapped expression‐level summaries and point biserial correlation scores to sort the samples based on a gene's expression level or by how dissimilar the read map profile is from a gene splice variant, to quickly identify samples with the strongest expression level or where alternative splicing might be occurring. …”
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    An ‘eFP‐Seq Browser’ for visualizing and exploring RNA sequencing data
  11. 5231
    “…Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. …”
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    Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey
  12. 5232
    “…Urine samples were first examined under a stereomicroscope; then, they were centrifuged and microscopically examined after a flotation test. As for foxes, the urinary bladders were opened and urine was collected and processed as above, while collected nematodes were identified at the species level. …”
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    Pearsonema spp. (Family Capillariidae, Order Enoplida) Infection in Domestic Carnivores in Central–Northern Italy and in a Red Fox Population from Central Italy
  13. 5233
    “…Isolation of taeniid eggs from faeces was performed by sieving/flotation technique, followed by DNA isolation, PCR and sequence analyses for species identification (gene target: small subunit of ribosomal RNA). …”
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    Occurrence of Echinococcus granulosus sensu lato and Other Taeniids in Bhutan
  14. 5234
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    Unraveling patients’ readiness in advance care planning conversations: a qualitative study as part of the ACTION Study
  15. 5235
    “…Next-generation sequencing (NGS) has become the gold standard for advanced microbiome analysis; however, 3rd generation real-time sequencing, such as Oxford Nanopore Technologies (ONT), enables rapid sequencing from several kilobases to >2 Mb with high resolution. Despite the wide availability and the enormous potential for clinical and translational applications, ONT is poorly standardized in terms of sampling and storage conditions, DNA extraction, library creation, and bioinformatic classification. …”
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    Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
  16. 5236
    “…A total of 495 faecal samples collected from pet, shelter, guard, working (police), and hunting dogs, as well as dogs from segregated Roma settlements between 2016 and 2021, were examined using flotation and molecular methods. Eggs of intestinal helminths were detected in 134 (27.1%) samples. …”
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    A Survey of Intestinal Helminths of Dogs in Slovakia with an Emphasis on Zoonotic Species
  17. 5237
    “…Abbreviations: m(6)A: N(6)-methyladenosine; ccRCC: clear cell renal cell carcinoma; DEGs: differentially expressed genes; NUF2: NUF2 component of NDC80 kinetochore complex; CDCA3: cell division cycle associated 3; CKAP2L: cytoskeleton associated protein 2 like; KIF14: kinesin family member 14; ASPM: assembly factor for spindle microtubules; METTL14: methyltransferase 14; OS: overall survival; FPKM: fragments per kilobase million; GEO: gene expression omnibus; TCGA: the Cancer Genome Atlas; RMA: robust multi-array average expression measure; WGCNA: weighted gene co-expression network analysis; GO: gene ontology; KEGG: kyoto encyclopedia of genes and genomes; ROC: receiver operating characteristic curve; AUC: area under the curve; RIP: RNA immunoprecipitation; qPCR: real-time quantitative PCR.…”
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    Analysis and verification of N(6)-methyladenosine-modified genes as novel biomarkers for clear cell renal cell carcinoma
  18. 5238
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    Delayed treatment with a tumor necrosis factor alpha blocker associated with worse outcomes in patients with spondyloarthritis: data from the Czech National Registry ATTRA
  19. 5239
    por Csomos, Krisztian, Ujhazi, Boglarka, Blazso, Peter, Herrera, Jose L., Tipton, Christopher M., Kawai, Tomoki, Gordon, Sumai, Ellison, Maryssa, Wu, Kevin, Stowell, Matthew, Haynes, Lauren, Cruz, Rachel, Zakota, Bence, Nguyen, Johnny, Altrich, Michelle, Geier, Christoph B., Sharapova, Svetlana, Dasso, Joseph F., Leiding, Jennifer W., Smith, Grace, Al-Herz, Waleed, de Barros Dorna, Mayra, Fadugba, Olajumoke, Fronkova, Eva, Kanderova, Veronika, Svaton, Michael, Henrickson, Sarah E., Hernandez, Joseph D., Kuijpers, Taco, Kandilarova, Snezhina Mihailova, Naumova, Elizaveta, Milota, Tomas, Sediva, Anna, Moshous, Despina, Neven, Benedicte, Saco, Tara, Sargur, Ravishankar, Savic, Sinisa, Sleasman, John, Sunkersett, Gauri, Ward, Brant R., Komatsu, Masanobu, Pittaluga, Stefania, Kumanovics, Attila, Butte, Manish J., Cancro, Michael P., Pillai, Shiv, Meffre, Eric, Notarangelo, Luigi D., Walter, Jolan E.
    Publicado 2022
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    Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet(+) B cells
  20. 5240
    “…Through the analysis of the FPKM (Fragments Per Kilobase of exon model per Million mapped fragments) values of the genes, we screened three peak egg-laying period-specific expressed genes: IHH, INHA, and CYP19A1. …”
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    Transcriptome Analysis of the Ovaries of Taihe Black-Bone Silky Fowls at Different Egg-Laying Stages
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