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5241por Asa, Bertha Fru, Shintouo, Cabirou Mounchili, Shey, Robert Adamu, Afoumbom, Mildred Tita, Siekeh, Nadia, Yoah, Adolf, Kah, Emmanuel, Ickowitz, Amy, Tata, Caleb Yengo, Asongalem, Emmanuel, Ghogomu, Stephen Mbigha“…Parasitological assessment was performed using a combination of direct microscopy flotation, sedimentation and centrifugation techniques. …”
Publicado 2022
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5242por Samorek-Pieróg, Małgorzata, Cencek, Tomasz, Łabuć, Emilia, Pac-Sosińska, Małgorzata, Pieróg, Mateusz, Korpysa-Dzirba, Weronika, Bełcik, Aneta, Bilska-Zając, Ewa, Karamon, Jacek“…RESULTS: From a total of 606 studies describing the occurrence of E. aerophilus, 141 articles from 38 countries worldwide were included in this meta-analysis, all of which presented results obtained mainly with flotation and necropsy. Due to the occurrence of E. aerophilus in many different species and different matrices (lungs and faeces), we decided to conduct the meta-analysis separately for each species with a given matrix. …”
Publicado 2023
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5243por Lin, Ching-Hung, Chen, BoJia, Chao, Day-Yu, Hsieh, Feng-Cheng, Yang, Chun-Chun, Hsu, Hsuan-Wei, Tam, Hon-Man-Herman, Wu, Hung-Yi“…The MHV-A59 full-length genomic cDNAs (~ 31 kilobases) were in vitro constructed to experimentally validate the origin of coronavirus DVG. …”
Publicado 2023
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5244por Xie, Hongbo M, Perin, Juan C, Schurr, Theodore G, Dulik, Matthew C, Zhadanov, Sergey I, Baur, Joseph A, King, Michael P, Place, Emily, Clarke, Colleen, Grauer, Michael, Schug, Jonathan, Santani, Avni, Albano, Anthony, Kim, Cecilia, Procaccio, Vincent, Hakonarson, Hakon, Gai, Xiaowu, Falk, Marni J“…We used this custom pipeline to analyze MitoChip v2.0 data from 24 DNA samples representing a broad range of tissue types (18 whole blood, 3 skeletal muscle, 3 cell lines), mutations (a 5.8 kilobase pair deletion and 6 known heteroplasmic mutations), and haplogroup origins. …”
Publicado 2011
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5245por Pope, Benjamin D., Ryba, Tyrone, Dileep, Vishnu, Yue, Feng, Wu, Weisheng, Denas, Olgert, Vera, Daniel L., Wang, Yanli, Hansen, R. Scott, Canfield, Theresa K., Thurman, Robert E., Cheng, Yong, Gülsoy, Günhan, Dennis, Jonathan H., Snyder, Michael P., Stamatoyannopoulos, John A., Taylor, James, Hardison, Ross C., Kahveci, Tamer, Ren, Bing, Gilbert, David M.“…In mammals, replication timing is cell-type-specific with at least half the genome switching replication timing during development, primarily in units of 400–800 kilobases (‘replication domains’), whose positions are preserved in different cell types, conserved between species, and appear to confine long-range effects of chromosome rearrangements(2,3,4,5,6,7). …”
Publicado 2014
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5246por Tsai, Chi-Chu, Wu, Keh-Ming, Chiang, Tzen-Yuh, Huang, Chun-Yen, Chou, Chang-Hung, Li, Shu-Ju, Chiang, Yu-Chung“…RESULTS: Transcriptome comparison between the vegetative propagation corms and juvenile tubers of G. elata revealed 703 differentially expressed unigenes, of which 298 and 405 unigenes were, respectively up-regulated (fold-change ≥ 2, q-value < 0.05, the trimmed mean of M-values (TMM)-normalized fragments per kilobase of transcript per Million mapped reads (FPKM) > 10) and down-regulated (fold-change ≤ 0.5, q-value <0.05, TMM-normalized FPKM > 10) in juvenile tubers. …”
Publicado 2016
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5247por Dapprich, Johannes, Ferriola, Deborah, Mackiewicz, Kate, Clark, Peter M., Rappaport, Eric, D’Arcy, Monica, Sasson, Ariella, Gai, Xiaowu, Schug, Jonathan, Kaestner, Klaus H., Monos, Dimitri“…While emerging sequencing platforms are capable of producing several kilobases-long reads, the fragment sizes generated by current DNA target enrichment technologies remain a limiting factor, producing DNA fragments generally shorter than 1 kbp. …”
Publicado 2016
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5248por Freixas Coutin, José A., Munholland, Seth, Silva, Anjali, Subedi, Sanjeena, Lukens, Lewis, Crosby, William L., Pauls, K. Peter, Bozzo, Gale G.“…RNA sequencing (RNA-seq) analysis was used to monitor changes in the seed coat transcriptome as a function of bean development, where transcript levels were measured as fragments per kilobase of exon per million fragments mapped. A total of 1336 genes were differentially expressed between darkening and non-darkening cranberry bean RILs. …”
Publicado 2017
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5249por Stapleton, Patrick, Eshaghi, Alireza, Chong-King, Eddie, Cardona, Mark, Masney, Steve, Li, Aimin, Gubbay, Jonathan, Patel, Samir“…Cell free viral extract underwent RT-PCR and subsequent PCR amplification using overlapping primer pairs to cover the entire 15 kilobase (kb) genome. The first 8 samples were amplified with 18 pairs of overlapping primers, which was reduced to 9 sets (average fragment size 1.9 kb, range 1.6–2.8 kb) for the final 12 samples. …”
Publicado 2017
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5250por Uiterwijk, Mathilde, Nijsse, Rolf, Kooyman, Frans N. J., Wagenaar, Jaap A., Mughini-Gras, Lapo, Ploeger, Harm W.“…Moreover, fecal samples were tested with centrifugation sedimentation flotation (CSF) coproscopical analysis for presence of gastrointestinal parasites. …”
Publicado 2019
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5251por Schulze, Vanessa, Lurz, Peter W. W., Ferrari, Nicola, Romeo, Claudia, Steele, Michael A., Marino, Shealyn, Mazzamuto, Maria Vittoria, Calvignac-Spencer, Sébastien, Schlottau, Kore, Beer, Martin, Ulrich, Rainer G., Ehlers, Bernhard“…Attempts to extend the HV DPOL sequences in upstream direction resulted in contiguous sequences of around 3.3 kilobase pairs for one BHV and two GHVs. Phylogenetic analysis allocated the novel squirrel PyVs to the genera Alpha- and Betapolyomavirus, the BHVs to the genus Muromegalovirus, and the GHVs to the genera Rhadinovirus and Macavirus. …”
Publicado 2020
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5252Detection of gastrointestinal parasitism at recreational canine sites in the USA: the DOGPARCS studypor Stafford, Kristina, Kollasch, Todd M., Duncan, Kathryn T., Horr, Stephanie, Goddu, Troy, Heinz-Loomer, Christine, Rumschlag, Anthony J., Ryan, William G., Sweet, Sarah, Little, Susan E.“…Samples were analyzed by coproantigen immunoassay (CAI) (Fecal Dx® and Giardia Test, IDEXX Laboratories, Inc.) and zinc sulfate centrifugal flotation (CF). Owners responded to a questionnaire on their dog’s signalment and use of heartworm/intestinal parasite control medications (HWCM). …”
Publicado 2020
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5253“…Glyma.09G008400 annotated as encoding an ethylene forming enzyme, ACC oxidase (ACO), was found to be highly overexpressed in the mi hilum region at 165 RPKMs (reads per kilobase per million mapped reads) compared to the standard line at just 0.03 RPKMs. …”
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5254por Jarosz, Wojciech, Durant, Jean-Francois, Irenge, Leonid Mwana Wa Bene, Fogt-Wyrwas, Renata, Mizgajska-Wiktor, Hanna, Gala, Jean-Luc“…The resulting optimal workflow for processing spiked soil samples was then tested on environmental soil samples and compared with the conventional flotation-centrifugation and microscopic examination of Toxocara eggs. …”
Publicado 2021
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5255por Gandasegui, Javier, Grau-Pujol, Berta, Cambra-Pelleja, María, Escola, Valdemiro, Demontis, Maria Antonietta, Cossa, Anelsio, Jamine, José Carlos, Balaña-Fouce, Rafael, van Lieshout, Lisette, Muñoz, José, Martínez-Valladares, María“…METHODS: Three different protocols for stool sample processing were compared in 19 T. trichiura-positive samples: fresh stool, egg concentration using metallic sieves with decreasing pore size, and egg concentration followed by flotation with saturated salt solution. Yield of each protocol was assessed by estimating the load of parasite DNA by real-time PCR. …”
Publicado 2021
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5256por Ekawasti, Fitrine, Nurcahyo, Raden Wisnu, Firdausy, Lintang Winantya, Wardhana, April Hari, Sawitri, Dyah Haryuningtyas, Prastowo, Joko, Priyowidodo, Dwi“…RESULTS: Screening examination using the sugar flotation modification method showed that Eimeria spp. were prevalent in Indonesia, as 65.4% of the bacterial strain was detected. …”
Publicado 2021
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5257por Deplanque, Dominique, Cviklinski, Stanislas, Bardou, Marc, Ader, Florence, Blanchard, Hervé, Barthélémy, Philippe, David, Isabelle, D’Ortenzio, Eric, Espérou, Hélène, Launay, Odile, Lazarevic, Milan, Lechat, Philippe, Lethiec, Françoise, Levy, Yves, Pérol, David, Rage, Virginie, Roustit, Matthieu, Thabut, Gabriel“…La pandémie de coronavirus disease-19 (COVID-19) a conduit au déploiement d’un effort de recherche académique et industriel sans précédent dont on peut regretter le caractère parfois redondant ainsi que le manque de pilotage tant national qu’international. Pourtant, force est de constater qu’à l’occasion de cette crise, les procédures réglementaires ont été adaptées de même que certains freins dans l’organisation de la recherche clinique ont pu être en partie levés pour contribuer au déploiement d’essais au plus près des patients et faciliter les modalités de suivi et de contrôle. …”
Publicado 2022
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5258por Adetula, Adeyinka A., Fan, Xinhao, Zhang, Yongsheng, Yao, Yilong, Yan, Junyu, Chen, Muya, Tang, Yijie, Liu, Yuwen, Yi, Guoqiang, Li, Kui, Tang, Zhonglin“…Abbreviations: A-to-I: Adenosine-to-inosine; ADAR: Adenosine deaminase acting on RNA; RES: RNA editing site; DEG: Differentially edited gene; DES: Differentially edited site; FDR: False discovery rate; GO: Gene Ontology; KEGG: Kyoto Encyclopaedia of Genes and Genomes; MDS cell: musclederived satellite cell; RPKM: Reads per kilobase of exon model in a gene per million mapped reads; UTR: Untranslated coding regions…”
Publicado 2021
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5259por Xiao, Senlin, Xing, Jingfeng, Nie, Tiange, Su, Aiguo, Zhang, Ruyang, Zhao, Yanxin, Song, Wei, Zhao, Jiuran“…In the mitochondrial genome of CMS-Sb, a 3.3-kilobase sequence containing nad1-exon1 was absent from the second copy of the 17-kb repeat region. …”
Publicado 2022
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5260por Wang, Honglei, Liu, Hui, Li, Liyan, Chen, Yingying, Liu, Zhaoyun, Li, Lijuan, Ding, Shaoxue, Ding, Kai, Fu, Rong“…The mRNAs with fragments per kilobase of exon model per million mapped fragments (FPKM) > 10 in at least 3 patients were selected, and experiments were performed to identify their upstream regulatory lncRNAs. …”
Publicado 2023
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