Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester

High-purity eicosapentaenoic acid (EPA) ethyl ester (EPA-EE) can be produced from an integrated technique consisting of saponification, ethyl esterification, urea complexation, molecular distillation and column separation. In order to improve the purity and inhibit oxidation, tea polyphenol palmitat...

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Autores principales: Ding, Xuyang, Liu, Fujun, Zheng, Rui, Pei, Xuechen, Wang, Ziye, Zhou, Dayong, Yin, Fawen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000512/
https://www.ncbi.nlm.nih.gov/pubmed/36900492
http://dx.doi.org/10.3390/foods12050975
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author Ding, Xuyang
Liu, Fujun
Zheng, Rui
Pei, Xuechen
Wang, Ziye
Zhou, Dayong
Yin, Fawen
author_facet Ding, Xuyang
Liu, Fujun
Zheng, Rui
Pei, Xuechen
Wang, Ziye
Zhou, Dayong
Yin, Fawen
author_sort Ding, Xuyang
collection PubMed
description High-purity eicosapentaenoic acid (EPA) ethyl ester (EPA-EE) can be produced from an integrated technique consisting of saponification, ethyl esterification, urea complexation, molecular distillation and column separation. In order to improve the purity and inhibit oxidation, tea polyphenol palmitate (TPP) was added before the procedure of ethyl esterification. Furthermore, through the optimization of process parameters, 2:1 (mass ratio of urea to fish oil, g/g), 6 h (crystallization time) and 4:1 (mass ratio of ethyl alcohol to urea, g/g) were found to be the optimum conditions in the procedure of urea complexation. Distillate (fraction collection), 115 °C (distillation temperature) and one stage (the number of stages) were found to be the optimum conditions for the procedure of molecular distillation. With the addition of TPP and the above optimum conditions, high-purity (96.95%) EPA-EE was finally obtained after column separation.
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spelling pubmed-100005122023-03-11 Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester Ding, Xuyang Liu, Fujun Zheng, Rui Pei, Xuechen Wang, Ziye Zhou, Dayong Yin, Fawen Foods Article High-purity eicosapentaenoic acid (EPA) ethyl ester (EPA-EE) can be produced from an integrated technique consisting of saponification, ethyl esterification, urea complexation, molecular distillation and column separation. In order to improve the purity and inhibit oxidation, tea polyphenol palmitate (TPP) was added before the procedure of ethyl esterification. Furthermore, through the optimization of process parameters, 2:1 (mass ratio of urea to fish oil, g/g), 6 h (crystallization time) and 4:1 (mass ratio of ethyl alcohol to urea, g/g) were found to be the optimum conditions in the procedure of urea complexation. Distillate (fraction collection), 115 °C (distillation temperature) and one stage (the number of stages) were found to be the optimum conditions for the procedure of molecular distillation. With the addition of TPP and the above optimum conditions, high-purity (96.95%) EPA-EE was finally obtained after column separation. MDPI 2023-02-25 /pmc/articles/PMC10000512/ /pubmed/36900492 http://dx.doi.org/10.3390/foods12050975 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Xuyang
Liu, Fujun
Zheng, Rui
Pei, Xuechen
Wang, Ziye
Zhou, Dayong
Yin, Fawen
Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title_full Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title_fullStr Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title_full_unstemmed Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title_short Effects of Addition of Tea Polyphenol Palmitate and Process Parameters on the Preparation of High-Purity EPA Ethyl Ester
title_sort effects of addition of tea polyphenol palmitate and process parameters on the preparation of high-purity epa ethyl ester
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10000512/
https://www.ncbi.nlm.nih.gov/pubmed/36900492
http://dx.doi.org/10.3390/foods12050975
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