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Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor
Modulation of the CXCL12–CXCR4 signaling axis is of the utmost importance due to its central involvement in several pathological disorders, including inflammatory diseases and cancer. Among the different currently available drugs that inhibit CXCR4 activation, motixafortide—a best-in-class antagonis...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001991/ https://www.ncbi.nlm.nih.gov/pubmed/36901829 http://dx.doi.org/10.3390/ijms24054393 |
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author | Rebolledo-Bustillo, Mariana Garcia-Gomez, David Dávila, Eliud Morales Castro, María Eugenia Caballero, Norma A. Melendez, Francisco J. Baizabal-Aguirre, Victor M. Sanchez-Gaytan, Brenda L. Perez-Aguilar, Jose Manuel |
author_facet | Rebolledo-Bustillo, Mariana Garcia-Gomez, David Dávila, Eliud Morales Castro, María Eugenia Caballero, Norma A. Melendez, Francisco J. Baizabal-Aguirre, Victor M. Sanchez-Gaytan, Brenda L. Perez-Aguilar, Jose Manuel |
author_sort | Rebolledo-Bustillo, Mariana |
collection | PubMed |
description | Modulation of the CXCL12–CXCR4 signaling axis is of the utmost importance due to its central involvement in several pathological disorders, including inflammatory diseases and cancer. Among the different currently available drugs that inhibit CXCR4 activation, motixafortide—a best-in-class antagonist of this GPCR receptor—has exhibited promising results in preclinical studies of pancreatic, breast, and lung cancers. However, detailed information on the interaction mechanism of motixafortide is still lacking. Here, we characterize the motixafortide/CXCR4 and CXCL12/CXCR4 protein complexes by using computational techniques including unbiased all-atom molecular dynamics simulations. Our microsecond-long simulations of the protein systems indicate that the agonist triggers changes associated with active-like GPCR conformations, while the antagonist favors inactive conformations of CXCR4. Detailed ligand–protein analysis indicates the importance of motixafortide’s six cationic residues, all of which established charge–charge interactions with acidic CXCR4 residues. Furthermore, two synthetic bulky chemical moieties of motixafortide work in tandem to restrict the conformations of important residues associated with CXCR4 activation. Our results not only elucidate the molecular mechanism by which motixafortide interacts with the CXCR4 receptor and stabilizes its inactive states, but also provide essential information to rationally design CXCR4 inhibitors that preserve the outstanding pharmacological features of motixafortide. |
format | Online Article Text |
id | pubmed-10001991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100019912023-03-11 Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor Rebolledo-Bustillo, Mariana Garcia-Gomez, David Dávila, Eliud Morales Castro, María Eugenia Caballero, Norma A. Melendez, Francisco J. Baizabal-Aguirre, Victor M. Sanchez-Gaytan, Brenda L. Perez-Aguilar, Jose Manuel Int J Mol Sci Article Modulation of the CXCL12–CXCR4 signaling axis is of the utmost importance due to its central involvement in several pathological disorders, including inflammatory diseases and cancer. Among the different currently available drugs that inhibit CXCR4 activation, motixafortide—a best-in-class antagonist of this GPCR receptor—has exhibited promising results in preclinical studies of pancreatic, breast, and lung cancers. However, detailed information on the interaction mechanism of motixafortide is still lacking. Here, we characterize the motixafortide/CXCR4 and CXCL12/CXCR4 protein complexes by using computational techniques including unbiased all-atom molecular dynamics simulations. Our microsecond-long simulations of the protein systems indicate that the agonist triggers changes associated with active-like GPCR conformations, while the antagonist favors inactive conformations of CXCR4. Detailed ligand–protein analysis indicates the importance of motixafortide’s six cationic residues, all of which established charge–charge interactions with acidic CXCR4 residues. Furthermore, two synthetic bulky chemical moieties of motixafortide work in tandem to restrict the conformations of important residues associated with CXCR4 activation. Our results not only elucidate the molecular mechanism by which motixafortide interacts with the CXCR4 receptor and stabilizes its inactive states, but also provide essential information to rationally design CXCR4 inhibitors that preserve the outstanding pharmacological features of motixafortide. MDPI 2023-02-23 /pmc/articles/PMC10001991/ /pubmed/36901829 http://dx.doi.org/10.3390/ijms24054393 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rebolledo-Bustillo, Mariana Garcia-Gomez, David Dávila, Eliud Morales Castro, María Eugenia Caballero, Norma A. Melendez, Francisco J. Baizabal-Aguirre, Victor M. Sanchez-Gaytan, Brenda L. Perez-Aguilar, Jose Manuel Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title | Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title_full | Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title_fullStr | Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title_full_unstemmed | Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title_short | Structural Basis of the Binding Mode of the Antineoplastic Compound Motixafortide (BL-8040) in the CXCR4 Chemokine Receptor |
title_sort | structural basis of the binding mode of the antineoplastic compound motixafortide (bl-8040) in the cxcr4 chemokine receptor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10001991/ https://www.ncbi.nlm.nih.gov/pubmed/36901829 http://dx.doi.org/10.3390/ijms24054393 |
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