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Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality
Mould development in foodstuffs is linked to both spoilage and the production of mycotoxins, provoking food quality and food safety concerns, respectively. The high-throughput technology proteomics applied to foodborne moulds is of great interest to address such issues. This review presents proteomi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003330/ https://www.ncbi.nlm.nih.gov/pubmed/36902140 http://dx.doi.org/10.3390/ijms24054709 |
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author | Álvarez, Micaela Andrade, María J. Núñez, Félix Rodríguez, Mar Delgado, Josué |
author_facet | Álvarez, Micaela Andrade, María J. Núñez, Félix Rodríguez, Mar Delgado, Josué |
author_sort | Álvarez, Micaela |
collection | PubMed |
description | Mould development in foodstuffs is linked to both spoilage and the production of mycotoxins, provoking food quality and food safety concerns, respectively. The high-throughput technology proteomics applied to foodborne moulds is of great interest to address such issues. This review presents proteomics approaches useful for boosting strategies to minimise the mould spoilage and the hazard related to mycotoxins in food. Metaproteomics seems to be the most effective method for mould identification despite the current problems related to the bioinformatics tool. More interestingly, different high resolution mass spectrometry tools are suitable for evaluating the proteome of foodborne moulds able to unveil the mould’s response under certain environmental conditions and the presence of biocontrol agents or antifungals, being sometimes combined with a method with limited ability to separate proteins, the two-dimensional gel electrophoresis. However, the matrix complexity, the high ranges of protein concentrations needed and the performing of multiple steps are some of the proteomics limitations for the application to foodborne moulds. To overcome some of these limitations, model systems have been developed and proteomics applied to other scientific fields, such as library-free data independent acquisition analyses, the implementation of ion mobility, and the evaluation of post-translational modifications, are expected to be gradually implemented in this field for avoiding undesirable moulds in foodstuffs. |
format | Online Article Text |
id | pubmed-10003330 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100033302023-03-11 Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality Álvarez, Micaela Andrade, María J. Núñez, Félix Rodríguez, Mar Delgado, Josué Int J Mol Sci Review Mould development in foodstuffs is linked to both spoilage and the production of mycotoxins, provoking food quality and food safety concerns, respectively. The high-throughput technology proteomics applied to foodborne moulds is of great interest to address such issues. This review presents proteomics approaches useful for boosting strategies to minimise the mould spoilage and the hazard related to mycotoxins in food. Metaproteomics seems to be the most effective method for mould identification despite the current problems related to the bioinformatics tool. More interestingly, different high resolution mass spectrometry tools are suitable for evaluating the proteome of foodborne moulds able to unveil the mould’s response under certain environmental conditions and the presence of biocontrol agents or antifungals, being sometimes combined with a method with limited ability to separate proteins, the two-dimensional gel electrophoresis. However, the matrix complexity, the high ranges of protein concentrations needed and the performing of multiple steps are some of the proteomics limitations for the application to foodborne moulds. To overcome some of these limitations, model systems have been developed and proteomics applied to other scientific fields, such as library-free data independent acquisition analyses, the implementation of ion mobility, and the evaluation of post-translational modifications, are expected to be gradually implemented in this field for avoiding undesirable moulds in foodstuffs. MDPI 2023-03-01 /pmc/articles/PMC10003330/ /pubmed/36902140 http://dx.doi.org/10.3390/ijms24054709 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Álvarez, Micaela Andrade, María J. Núñez, Félix Rodríguez, Mar Delgado, Josué Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title | Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title_full | Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title_fullStr | Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title_full_unstemmed | Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title_short | Proteomics as a New-Generation Tool for Studying Moulds Related to Food Safety and Quality |
title_sort | proteomics as a new-generation tool for studying moulds related to food safety and quality |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10003330/ https://www.ncbi.nlm.nih.gov/pubmed/36902140 http://dx.doi.org/10.3390/ijms24054709 |
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