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Development of a New Route for the Immobilization of Unmodified Single-Stranded DNA on Chitosan Beads and Detection of Released Guanine after Hydrolysis

In this work, chitosan beads were used as a cost-effective platform for the covalent immobilization of unmodified single-stranded DNA, using glutaraldehyde as a cross-linking agent. The immobilized DNA capture probe was hybridized in the presence of miRNA-222 as a complementary sequence. The target...

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Detalles Bibliográficos
Autores principales: Chahri, Ikram, Karrat, Abdelhafid, Mohammadi, Hasna, Amine, Aziz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004340/
https://www.ncbi.nlm.nih.gov/pubmed/36903335
http://dx.doi.org/10.3390/molecules28052088