Cargando…

General Strategies for RNA X-ray Crystallography

An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal con...

Descripción completa

Detalles Bibliográficos
Autores principales: Jackson, Ryland W., Smathers, Claire M., Robart, Aaron R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004510/
https://www.ncbi.nlm.nih.gov/pubmed/36903357
http://dx.doi.org/10.3390/molecules28052111
_version_ 1784904851076939776
author Jackson, Ryland W.
Smathers, Claire M.
Robart, Aaron R.
author_facet Jackson, Ryland W.
Smathers, Claire M.
Robart, Aaron R.
author_sort Jackson, Ryland W.
collection PubMed
description An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice.
format Online
Article
Text
id pubmed-10004510
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100045102023-03-11 General Strategies for RNA X-ray Crystallography Jackson, Ryland W. Smathers, Claire M. Robart, Aaron R. Molecules Review An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice. MDPI 2023-02-23 /pmc/articles/PMC10004510/ /pubmed/36903357 http://dx.doi.org/10.3390/molecules28052111 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Jackson, Ryland W.
Smathers, Claire M.
Robart, Aaron R.
General Strategies for RNA X-ray Crystallography
title General Strategies for RNA X-ray Crystallography
title_full General Strategies for RNA X-ray Crystallography
title_fullStr General Strategies for RNA X-ray Crystallography
title_full_unstemmed General Strategies for RNA X-ray Crystallography
title_short General Strategies for RNA X-ray Crystallography
title_sort general strategies for rna x-ray crystallography
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004510/
https://www.ncbi.nlm.nih.gov/pubmed/36903357
http://dx.doi.org/10.3390/molecules28052111
work_keys_str_mv AT jacksonrylandw generalstrategiesforrnaxraycrystallography
AT smathersclairem generalstrategiesforrnaxraycrystallography
AT robartaaronr generalstrategiesforrnaxraycrystallography