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General Strategies for RNA X-ray Crystallography
An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal con...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004510/ https://www.ncbi.nlm.nih.gov/pubmed/36903357 http://dx.doi.org/10.3390/molecules28052111 |
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author | Jackson, Ryland W. Smathers, Claire M. Robart, Aaron R. |
author_facet | Jackson, Ryland W. Smathers, Claire M. Robart, Aaron R. |
author_sort | Jackson, Ryland W. |
collection | PubMed |
description | An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice. |
format | Online Article Text |
id | pubmed-10004510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100045102023-03-11 General Strategies for RNA X-ray Crystallography Jackson, Ryland W. Smathers, Claire M. Robart, Aaron R. Molecules Review An extremely small proportion of the X-ray crystal structures deposited in the Protein Data Bank are of RNA or RNA–protein complexes. This is due to three main obstacles to the successful determination of RNA structure: (1) low yields of pure, properly folded RNA; (2) difficulty creating crystal contacts due to low sequence diversity; and (3) limited methods for phasing. Various approaches have been developed to address these obstacles, such as native RNA purification, engineered crystallization modules, and incorporation of proteins to assist in phasing. In this review, we will discuss these strategies and provide examples of how they are used in practice. MDPI 2023-02-23 /pmc/articles/PMC10004510/ /pubmed/36903357 http://dx.doi.org/10.3390/molecules28052111 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Jackson, Ryland W. Smathers, Claire M. Robart, Aaron R. General Strategies for RNA X-ray Crystallography |
title | General Strategies for RNA X-ray Crystallography |
title_full | General Strategies for RNA X-ray Crystallography |
title_fullStr | General Strategies for RNA X-ray Crystallography |
title_full_unstemmed | General Strategies for RNA X-ray Crystallography |
title_short | General Strategies for RNA X-ray Crystallography |
title_sort | general strategies for rna x-ray crystallography |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10004510/ https://www.ncbi.nlm.nih.gov/pubmed/36903357 http://dx.doi.org/10.3390/molecules28052111 |
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