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Acyl carrier protein tag can enhance tobacco etch virus protease stability and promote its covalent immobilisation
ABSTRACT: Fusion expression is widely employed to enhance the solubility of recombinant proteins. However, removal of the fusion tag is often required due to its potential impact on the structure and activity of passenger proteins. Tobacco etch virus (TEV) protease is widely used for this purpose du...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006060/ https://www.ncbi.nlm.nih.gov/pubmed/36763116 http://dx.doi.org/10.1007/s00253-023-12377-8 |