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Acyl carrier protein tag can enhance tobacco etch virus protease stability and promote its covalent immobilisation

ABSTRACT: Fusion expression is widely employed to enhance the solubility of recombinant proteins. However, removal of the fusion tag is often required due to its potential impact on the structure and activity of passenger proteins. Tobacco etch virus (TEV) protease is widely used for this purpose du...

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Detalles Bibliográficos
Autores principales:	Li, Xuefeng, Huang, Jiahua, Zhou, Junjie, Sun, Changsheng, Zheng, Yujiao, Wang, Yuan, Zhu, Jin, Wang, Shengbin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2023
Materias:	Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10006060/ https://www.ncbi.nlm.nih.gov/pubmed/36763116 http://dx.doi.org/10.1007/s00253-023-12377-8

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