A Facile Fluorometric Assay of Orotate Phosphoribosyltransferase Activity Using a Selective Fluorogenic Reaction for Orotic Acid

Orotate phosphoribosyltransferase (OPRT) exists as a bifunctional enzyme, uridine 5′-monophosphate synthase, in mammalian cells and plays an important role in pyrimidine biosynthesis. Measuring OPRT activity has been considered important for understanding biological events and development of molecul...

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Detalles Bibliográficos
Autores principales: Shibata, Takayuki, Narita, Tomohiro, Suto, Yutaka, Yasmin, Hasina, Kabashima, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007123/
https://www.ncbi.nlm.nih.gov/pubmed/36904710
http://dx.doi.org/10.3390/s23052507

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10007123/
https://www.ncbi.nlm.nih.gov/pubmed/36904710
http://dx.doi.org/10.3390/s23052507

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