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Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization

Reaching sub-millisecond 3D tracking of individual molecules in living cells would enable direct measurements of diffusion-limited macromolecular interactions under physiological conditions. Here, we present a 3D tracking principle that approaches the relevant regime. The method is based on the true...

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Detalles Bibliográficos
Autores principales: Amselem, Elias, Broadwater, Bo, Hävermark, Tora, Johansson, Magnus, Elf, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008558/
https://www.ncbi.nlm.nih.gov/pubmed/36906676
http://dx.doi.org/10.1038/s41467-023-36879-1