Cargando…

Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl(2) nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients

Programmed cell death ligand 1 protein-positive (PD-L1(+)) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1(+) exosomes is still a challenge in clinical application...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang, Luyue, Wu, Haiping, Chen, Rui, Sun, Xiaoqing, Yang, Yi, Huang, Changwu, Ding, Shijia, Liu, Changjin, Cheng, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008610/
https://www.ncbi.nlm.nih.gov/pubmed/36906540
http://dx.doi.org/10.1186/s12951-023-01845-y
Descripción
Sumario:Programmed cell death ligand 1 protein-positive (PD-L1(+)) exosomes have been found to be a potential biomarker for the diagnosis of non-small cell lung cancer (NSCLC). However, the development of highly sensitive detection technique for PD-L1(+) exosomes is still a challenge in clinical applications. Herein, a sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl(2) nanowires (NWs) was designed for the detection of PD-L1(+) exosomes. The excellent peroxidase-like catalytic activity of PdCuB MNs and the high conductivity of Au@CuCl(2) NWs endow the fabricated aptasensor with intense electrochemical signal, thus enabling the detection of low abundance exosomes. The analytical results revealed that the aptasensor maintained favorable linearity over a wide concentration range of 6 orders of magnitude and reached a low detection limit of 36 particles/mL. The aptasensor is successfully applied to the analysis of complex serum samples and achieves the accurate identification of clinical NSCLC patients. Overall, the developed electrochemical aptasensor provides a powerful tool for early diagnosis of NSCLC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-01845-y.