Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays

Myosin expression and purification is important for mechanistic insights into normal function and mutation induced changes. The latter is particularly important for striated muscle myosin II where mutations cause several debilitating diseases. However, the heavy chain of this myosin is challenging t...

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Detalles Bibliográficos
Autores principales: Velayuthan, Lok Priya, Moretto, Luisa, Tågerud, Sven, Ušaj, Marko, Månsson, Alf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008826/
https://www.ncbi.nlm.nih.gov/pubmed/36907906
http://dx.doi.org/10.1038/s41598-023-30576-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008826/
https://www.ncbi.nlm.nih.gov/pubmed/36907906
http://dx.doi.org/10.1038/s41598-023-30576-1

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