Enrichment of transgene integrations by transient CRISPR activation of a silent reporter gene

CRISPR-Cas-mediated site-specific integration of transgenes by homology-directed repair (HDR) is challenging, especially in primary cells, where inferior editing efficiency may impede the development of gene- and cellular therapies. Various strategies for enrichment of cells with transgene integrati...

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Detalles Bibliográficos
Autores principales: Mikkelsen, Nanna S., Hernandez, Sabina S., Jensen, Trine I., Schneller, Jessica L., Bak, Rasmus O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009645/
https://www.ncbi.nlm.nih.gov/pubmed/36922985
http://dx.doi.org/10.1016/j.omtm.2023.02.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009645/
https://www.ncbi.nlm.nih.gov/pubmed/36922985
http://dx.doi.org/10.1016/j.omtm.2023.02.010

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