A protocol for single nucleus RNA-seq from frozen skeletal muscle

Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, an...

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Detalles Bibliográficos
Autores principales: Soule, Tyler GB, Pontifex, Carly S, Rosin, Nicole, Joel, Matthew M, Lee, Sukyoung, Nguyen, Minh Dang, Chhibber, Sameer, Pfeffer, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011611/
https://www.ncbi.nlm.nih.gov/pubmed/36914268
http://dx.doi.org/10.26508/lsa.202201806

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011611/
https://www.ncbi.nlm.nih.gov/pubmed/36914268
http://dx.doi.org/10.26508/lsa.202201806

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