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A protocol for single nucleus RNA-seq from frozen skeletal muscle
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, an...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011611/ https://www.ncbi.nlm.nih.gov/pubmed/36914268 http://dx.doi.org/10.26508/lsa.202201806 |
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author | Soule, Tyler GB Pontifex, Carly S Rosin, Nicole Joel, Matthew M Lee, Sukyoung Nguyen, Minh Dang Chhibber, Sameer Pfeffer, Gerald |
author_facet | Soule, Tyler GB Pontifex, Carly S Rosin, Nicole Joel, Matthew M Lee, Sukyoung Nguyen, Minh Dang Chhibber, Sameer Pfeffer, Gerald |
author_sort | Soule, Tyler GB |
collection | PubMed |
description | Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by single-nucleus RNA sequencing. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease. |
format | Online Article Text |
id | pubmed-10011611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-100116112023-03-15 A protocol for single nucleus RNA-seq from frozen skeletal muscle Soule, Tyler GB Pontifex, Carly S Rosin, Nicole Joel, Matthew M Lee, Sukyoung Nguyen, Minh Dang Chhibber, Sameer Pfeffer, Gerald Life Sci Alliance Methods Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, and cost-effective method to analyze frozen human skeletal muscle by single-nucleus RNA sequencing. This method yields all expected cell types for human skeletal muscle and works on tissue frozen for long periods of time and with significant pathological changes. Our method is ideal for studying banked samples with the intention of studying human muscle disease. Life Science Alliance LLC 2023-03-13 /pmc/articles/PMC10011611/ /pubmed/36914268 http://dx.doi.org/10.26508/lsa.202201806 Text en © 2023 Soule et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Methods Soule, Tyler GB Pontifex, Carly S Rosin, Nicole Joel, Matthew M Lee, Sukyoung Nguyen, Minh Dang Chhibber, Sameer Pfeffer, Gerald A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title | A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title_full | A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title_fullStr | A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title_full_unstemmed | A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title_short | A protocol for single nucleus RNA-seq from frozen skeletal muscle |
title_sort | protocol for single nucleus rna-seq from frozen skeletal muscle |
topic | Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011611/ https://www.ncbi.nlm.nih.gov/pubmed/36914268 http://dx.doi.org/10.26508/lsa.202201806 |
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