Cargando…
A protocol for single nucleus RNA-seq from frozen skeletal muscle
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA sequencing because of their large, multinucleated nature. Here, we report a novel, reliable, an...
Autores principales: | Soule, Tyler GB, Pontifex, Carly S, Rosin, Nicole, Joel, Matthew M, Lee, Sukyoung, Nguyen, Minh Dang, Chhibber, Sameer, Pfeffer, Gerald |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011611/ https://www.ncbi.nlm.nih.gov/pubmed/36914268 http://dx.doi.org/10.26508/lsa.202201806 |
Ejemplares similares
-
Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies
por: Schmøkel, Sofie S., et al.
Publicado: (2023) -
FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants
por: Long, Yanping, et al.
Publicado: (2021) -
Single-nucleus RNA-seq of differentiating human myoblasts reveals the extent of fate heterogeneity
por: Zeng, Weihua, et al.
Publicado: (2016) -
SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology
por: Abdelmoez, Mahmoud N., et al.
Publicado: (2018) -
Identification of bona fide B2 SINE retrotransposon transcription through single-nucleus RNA-seq of the mouse hippocampus
por: Linker, Sara B., et al.
Publicado: (2020)