Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability

A carboxylesterase derived from Sulfolobus solfataricus P1 was immobilized onto an epoxy-activated Sepharose resin via non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The inco...

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Detalles Bibliográficos
Autores principales: Switzer, Hannah J., Howard, Christina A., Halonski, John F., Peairs, Emily M., Smith, Nolan, Zamecnik, Maddy P., Verma, Sanjana, Young, Douglas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012417/
https://www.ncbi.nlm.nih.gov/pubmed/36926306
http://dx.doi.org/10.1039/d3ra00392b

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012417/
https://www.ncbi.nlm.nih.gov/pubmed/36926306
http://dx.doi.org/10.1039/d3ra00392b

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