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Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability
A carboxylesterase derived from Sulfolobus solfataricus P1 was immobilized onto an epoxy-activated Sepharose resin via non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The inco...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10012417/ https://www.ncbi.nlm.nih.gov/pubmed/36926306 http://dx.doi.org/10.1039/d3ra00392b |