Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage

Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR–Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the cryo-EM structure and functional studies of t...

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Detalles Bibliográficos
Autores principales: Jianwei, Li, Jobichen, Chacko, Machida, Satoru, Meng, Sun, Read, Randy J., Hongying, Chen, Jian, Shi, Yuan, Yuren Adam, Sivaraman, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013913/
https://www.ncbi.nlm.nih.gov/pubmed/36917574
http://dx.doi.org/10.1371/journal.pbio.3002023

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013913/
https://www.ncbi.nlm.nih.gov/pubmed/36917574
http://dx.doi.org/10.1371/journal.pbio.3002023

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