Cargando…

Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage

Cas12a is a programmable nuclease for adaptive immunity against invading nucleic acids in CRISPR–Cas systems. Here, we report the crystal structures of apo Cas12a from Lachnospiraceae bacterium MA2020 (Lb2) and the Lb2Cas12a+crRNA complex, as well as the cryo-EM structure and functional studies of t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jianwei, Li, Jobichen, Chacko, Machida, Satoru, Meng, Sun, Read, Randy J., Hongying, Chen, Jian, Shi, Yuan, Yuren Adam, Sivaraman, J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10013913/ https://www.ncbi.nlm.nih.gov/pubmed/36917574 http://dx.doi.org/10.1371/journal.pbio.3002023

Ejemplares similares

Conformational Dynamics and Cleavage Sites of Cas12a Are Modulated by Complementarity between crRNA and DNA
por: Zhang, Lujia, et al.
Publicado: (2019)

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus
por: Karvelis, Tautvydas, et al.
Publicado: (2013)

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
por: Nguyen, Long T., et al.
Publicado: (2020)

Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicus
por: Peng, Wenfang, et al.
Publicado: (2013)

Author Correction: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
por: Nguyen, Long T., et al.
Publicado: (2020)

CRISPRTarget: Bioinformatic prediction and analysis of crRNA targets
por: Biswas, Ambarish, et al.
Publicado: (2013)

FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella
por: Cheng, Peipei, et al.
Publicado: (2021)

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
por: Hu, Menglu, et al.
Publicado: (2022)

Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a
por: Sinan, Selma, et al.
Publicado: (2023)

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA
por: Li, Yingjun, et al.
Publicado: (2017)

Features of CRISPR-Cas Regulation Key to Highly Efficient and Temporally-Specific crRNA Production
por: Rodic, Andjela, et al.
Publicado: (2017)

Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato
por: Vu, Tien Van, et al.
Publicado: (2021)

Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA
por: Gao, Zongliang, et al.
Publicado: (2018)

Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
por: Teng, Fei, et al.
Publicado: (2019)

Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles
por: Semikolenova, Olga, et al.
Publicado: (2021)

Ringhalexin from Hemachatus haemachatus: A novel inhibitor of extrinsic tenase complex
por: Barnwal, Bhaskar, et al.
Publicado: (2016)

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
por: Park, Hyo Min, et al.
Publicado: (2018)

Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion
por: Chow, Ryan D., et al.
Publicado: (2018)

Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
por: Ishibashi, Riki, et al.
Publicado: (2020)

Biosensing bacterial 16S rDNA by microchip electrophoresis combined with a CRISPR system based on real-time crRNA/Cas12a formation
por: Luo, Feifei, et al.
Publicado: (2022)

New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
por: Misiurina, Mariia A., et al.
Publicado: (2022)

DNA Cleavage, Cytotoxic Activities, and Antimicrobial Studies of Ternary Copper(II) Complexes of Isoxazole Schiff Base and Heterocyclic Compounds
por: Chityala, Vijay Kumar, et al.
Publicado: (2014)

Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers
por: Pica, Andrea, et al.
Publicado: (2017)

Probing the stability of the SpCas9–DNA complex after cleavage
por: Aldag, Pierre, et al.
Publicado: (2021)

CRISPRstrand: predicting repeat orientations to determine the crRNA-encoding strand at CRISPR loci
por: Alkhnbashi, Omer S., et al.
Publicado: (2014)

Enhanced FnCas12a-Mediated Targeted Mutagenesis Using crRNA With Altered Target Length in Rice
por: Negishi, Katsuya, et al.
Publicado: (2020)

The structure of a Type III-A CRISPR-Cas effector complex reveals conserved and idiosyncratic contacts to target RNA and crRNA among Type III-A systems
por: Paraan, Mohammadreza, et al.
Publicado: (2023)

Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA
por: Moon, Jeong, et al.
Publicado: (2023)

Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA
por: Gao, Zongliang, et al.
Publicado: (2020)

The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
por: Nasef, Mohamed, et al.
Publicado: (2022)

Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation
por: Su, Hang, et al.
Publicado: (2023)

Structures of HIV-1 RT-RNA/DNA ternary complexes with dATP and nevirapine reveal conformational flexibility of RNA/DNA: insights into requirements for RNase H cleavage
por: Das, Kalyan, et al.
Publicado: (2014)

Crystal structures of ternary complexes of archaeal B-family DNA polymerases
por: Kropp, Heike M., et al.
Publicado: (2017)

Drug-Induced Conformational Population Shifts in Topoisomerase-DNA Ternary Complexes
por: Huang, Nan-Lan, et al.
Publicado: (2014)

RNA-activated DNA cleavage by the Type III-B CRISPR–Cas effector complex
por: Estrella, Michael A., et al.
Publicado: (2016)

Hairpin‐Spacer crRNA‐Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification
por: Ke, Yuqing, et al.
Publicado: (2021)

A rapid and efficient platform for antiviral crRNA screening using CRISPR-Cas13a-based nucleic acid detection
por: Yang, Lan, et al.
Publicado: (2023)

In Vitro CRISPR-Cas12a-Based Detection of Cancer-Associated TP53 Hotspot Mutations Beyond the crRNA Seed Region
por: Kohabir, Kavish A.V., et al.
Publicado: (2023)

Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array
por: Zetsche, Bernd, et al.
Publicado: (2016)

Full-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State
por: Du, Wenhao, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_tmll7mna8aa2680knbp1c0fb6i