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High-yield vesicle-packaged recombinant protein production from E. coli

We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins...

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Detalles Bibliográficos
Autores principales: Eastwood, Tara A., Baker, Karen, Streather, Bree R., Allen, Nyasha, Wang, Lin, Botchway, Stanley W., Brown, Ian R., Hiscock, Jennifer R., Lennon, Christopher, Mulvihill, Daniel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014274/
https://www.ncbi.nlm.nih.gov/pubmed/36936078
http://dx.doi.org/10.1016/j.crmeth.2023.100396