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Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks

Diarrhea is a severe bovine disease, globally prevalent in farm animals with a decrease in milk production and a low fertility rate. Cryptosporidium spp. are important zoonotic agents of bovine diarrhea. However, little is known about microbiota and short-chain fatty acids (SCFAs) changes in yaks in...

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Autores principales: Dong, Hailong, Chen, Xiushuang, Zhao, Xiaoxiao, Zhao, Chenxi, Mehmood, Khalid, Kulyar, Muhammad Fakhar-e-Alam, Bhutta, Zeeshan Ahmad, Zeng, Jiangyong, Nawaz, Shah, Wu, Qingxia, Li, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014559/
https://www.ncbi.nlm.nih.gov/pubmed/36936759
http://dx.doi.org/10.3389/fcimb.2023.1105126
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author Dong, Hailong
Chen, Xiushuang
Zhao, Xiaoxiao
Zhao, Chenxi
Mehmood, Khalid
Kulyar, Muhammad Fakhar-e-Alam
Bhutta, Zeeshan Ahmad
Zeng, Jiangyong
Nawaz, Shah
Wu, Qingxia
Li, Kun
author_facet Dong, Hailong
Chen, Xiushuang
Zhao, Xiaoxiao
Zhao, Chenxi
Mehmood, Khalid
Kulyar, Muhammad Fakhar-e-Alam
Bhutta, Zeeshan Ahmad
Zeng, Jiangyong
Nawaz, Shah
Wu, Qingxia
Li, Kun
author_sort Dong, Hailong
collection PubMed
description Diarrhea is a severe bovine disease, globally prevalent in farm animals with a decrease in milk production and a low fertility rate. Cryptosporidium spp. are important zoonotic agents of bovine diarrhea. However, little is known about microbiota and short-chain fatty acids (SCFAs) changes in yaks infected with Cryptosporidium spp. Therefore, we performed 16S rRNA sequencing and detected the concentrations of SCFAs in Cryptosporidium-infected yaks. Results showed that over 80,000 raw and 70,000 filtered sequences were prevalent in yak samples. Shannon (p<0.01) and Simpson (p<0.01) were both significantly higher in Cryptosporidium-infected yaks. A total of 1072 amplicon sequence variants were shared in healthy and infected yaks. There were 11 phyla and 58 genera that differ significantly between the two yak groups. A total of 235 enzymes with a significant difference in abundance (p<0.001) were found between healthy and infected yaks. KEGG L3 analysis discovered that the abundance of 43 pathways was significantly higher, while 49 pathways were significantly lower in Cryptosporidium-infected yaks. The concentration of acetic acid (p<0.05), propionic acid (p<0.05), isobutyric acid (p<0.05), butyric acid (p<0.05), and isovaleric acid was noticeably lower in infected yaks, respectively. The findings of the study revealed that Cryptosporidium infection causes gut dysbiosis and results in a significant drop in the SCFAs concentrations in yaks with severe diarrhea, which may give new insights regarding the prevention and treatment of diarrhea in livestock.
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spelling pubmed-100145592023-03-16 Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks Dong, Hailong Chen, Xiushuang Zhao, Xiaoxiao Zhao, Chenxi Mehmood, Khalid Kulyar, Muhammad Fakhar-e-Alam Bhutta, Zeeshan Ahmad Zeng, Jiangyong Nawaz, Shah Wu, Qingxia Li, Kun Front Cell Infect Microbiol Cellular and Infection Microbiology Diarrhea is a severe bovine disease, globally prevalent in farm animals with a decrease in milk production and a low fertility rate. Cryptosporidium spp. are important zoonotic agents of bovine diarrhea. However, little is known about microbiota and short-chain fatty acids (SCFAs) changes in yaks infected with Cryptosporidium spp. Therefore, we performed 16S rRNA sequencing and detected the concentrations of SCFAs in Cryptosporidium-infected yaks. Results showed that over 80,000 raw and 70,000 filtered sequences were prevalent in yak samples. Shannon (p<0.01) and Simpson (p<0.01) were both significantly higher in Cryptosporidium-infected yaks. A total of 1072 amplicon sequence variants were shared in healthy and infected yaks. There were 11 phyla and 58 genera that differ significantly between the two yak groups. A total of 235 enzymes with a significant difference in abundance (p<0.001) were found between healthy and infected yaks. KEGG L3 analysis discovered that the abundance of 43 pathways was significantly higher, while 49 pathways were significantly lower in Cryptosporidium-infected yaks. The concentration of acetic acid (p<0.05), propionic acid (p<0.05), isobutyric acid (p<0.05), butyric acid (p<0.05), and isovaleric acid was noticeably lower in infected yaks, respectively. The findings of the study revealed that Cryptosporidium infection causes gut dysbiosis and results in a significant drop in the SCFAs concentrations in yaks with severe diarrhea, which may give new insights regarding the prevention and treatment of diarrhea in livestock. Frontiers Media S.A. 2023-03-01 /pmc/articles/PMC10014559/ /pubmed/36936759 http://dx.doi.org/10.3389/fcimb.2023.1105126 Text en Copyright © 2023 Dong, Chen, Zhao, Zhao, Mehmood, Kulyar, Bhutta, Zeng, Nawaz, Wu and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Dong, Hailong
Chen, Xiushuang
Zhao, Xiaoxiao
Zhao, Chenxi
Mehmood, Khalid
Kulyar, Muhammad Fakhar-e-Alam
Bhutta, Zeeshan Ahmad
Zeng, Jiangyong
Nawaz, Shah
Wu, Qingxia
Li, Kun
Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title_full Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title_fullStr Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title_full_unstemmed Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title_short Intestine microbiota and SCFAs response in naturally Cryptosporidium-infected plateau yaks
title_sort intestine microbiota and scfas response in naturally cryptosporidium-infected plateau yaks
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014559/
https://www.ncbi.nlm.nih.gov/pubmed/36936759
http://dx.doi.org/10.3389/fcimb.2023.1105126
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