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Strategies for optimizing CITE-seq for human islets and other tissues

Defining the immunological landscape of human tissue is an important area of research, but challenges include the impact of tissue disaggregation on cell phenotypes and the low abundance of immune cells in many tissues. Here, we describe methods to troubleshoot and standardize Cellular Indexing of T...

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Detalles Bibliográficos
Autores principales: Colpitts, Sarah J., Budd, Matthew A., Monajemi, Mahdis, Reid, Kyle T., Murphy, Julia M., Ivison, Sabine, Verchere, C. Bruce, Levings, Megan K., Crome, Sarah Q.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10014726/
https://www.ncbi.nlm.nih.gov/pubmed/36936943
http://dx.doi.org/10.3389/fimmu.2023.1107582