In vitro characterization of a pAgo nuclease TtdAgo from Thermococcus thioreducens and evaluation of its effect in vivo

In spite of the development of genome-editing tools using CRISPR–Cas systems, highly efficient and effective genome-editing tools are still needed that use novel programmable nucleases such as Argonaute (Ago) proteins to accelerate the construction of microbial cell factories. In this study, a proka...

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Detalles Bibliográficos
Autores principales: Tang, Ying, Wang, Fei, Wang, Yi, Wang, Yuwei, Liu, Yang, Chen, Zhizhao, Li, Wenqiang, Yang, Shihui, Ma, Lixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017986/
https://www.ncbi.nlm.nih.gov/pubmed/36937752
http://dx.doi.org/10.3389/fbioe.2023.1142637

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10017986/
https://www.ncbi.nlm.nih.gov/pubmed/36937752
http://dx.doi.org/10.3389/fbioe.2023.1142637

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