Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic substitutions

CRISPR nucleases can introduce double-stranded DNA breaks in genomes at positions specified by guide RNAs. When repaired by the cell, this may result in the introduction of insertions and deletions or nucleotide substitutions provided by exogenous DNA donors. However, cellular repair can also result...

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Detalles Bibliográficos
Autores principales: Lackner, Martin, Helmbrecht, Nelly, Pääbo, Svante, Riesenberg, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018342/
https://www.ncbi.nlm.nih.gov/pubmed/36620901
http://dx.doi.org/10.1093/nar/gkac1254

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018342/
https://www.ncbi.nlm.nih.gov/pubmed/36620901
http://dx.doi.org/10.1093/nar/gkac1254

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