High-throughput engineering of cytoplasmic- and nuclear-replicating large dsDNA viruses by CRISPR/Cas9

The application of CRISPR/Cas9 to improve genome engineering efficiency for large dsDNA viruses has been extensively described, but a robust and versatile method for high-throughput generation of marker-free recombinants for a desired locus has not yet been reported. Cytoplasmic-replicating viruses...

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Detalles Bibliográficos
Autores principales: López-Muñoz, Alberto Domingo, Rastrojo, Alberto, Martín, Rocío, Alcami, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2022
Materias:
Animal
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10019086/
https://www.ncbi.nlm.nih.gov/pubmed/36260063
http://dx.doi.org/10.1099/jgv.0.001797

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10019086/
https://www.ncbi.nlm.nih.gov/pubmed/36260063
http://dx.doi.org/10.1099/jgv.0.001797

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