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Realizing the value in “non-standard” parts of the qPCR standard curve by integrating fundamentals of quantitative microbiology

The real-time polymerase chain reaction (PCR), commonly known as quantitative PCR (qPCR), is increasingly common in environmental microbiology applications. During the COVID-19 pandemic, qPCR combined with reverse transcription (RT-qPCR) has been used to detect and quantify SARS-CoV-2 in clinical di...

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Detalles Bibliográficos
Autores principales: Schmidt, Philip J., Acosta, Nicole, Chik, Alex H. S., D’Aoust, Patrick M., Delatolla, Robert, Dhiyebi, Hadi A., Glier, Melissa B., Hubert, Casey R. J., Kopetzky, Jennifer, Mangat, Chand S., Pang, Xiao-Li, Peterson, Shelley W., Prystajecky, Natalie, Qiu, Yuanyuan, Servos, Mark R., Emelko, Monica B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020645/
https://www.ncbi.nlm.nih.gov/pubmed/36937263
http://dx.doi.org/10.3389/fmicb.2023.1048661