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Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis
BACKGROUND: Long non‐coding RNA (lncRNA) ATB belongs to an active modulator in multiple cancers, but its expression along with potential underlying non‐small cell lung cancer (NSCLC) is obscure. Our study aimed to investigate the role and potential mechanism of LncRNA ATB in NSCLC. METHODS: LncRNA A...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020841/ https://www.ncbi.nlm.nih.gov/pubmed/36806318 http://dx.doi.org/10.1002/jcla.24822 |
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author | Sun, Shifang Zou, Yifan Xu, Ningjie Wang, Kaiyue Rong, Shanshan Lv, Jiarong Hu, Bin Mai, Yifeng Zhu, Decai Ding, Liren |
author_facet | Sun, Shifang Zou, Yifan Xu, Ningjie Wang, Kaiyue Rong, Shanshan Lv, Jiarong Hu, Bin Mai, Yifeng Zhu, Decai Ding, Liren |
author_sort | Sun, Shifang |
collection | PubMed |
description | BACKGROUND: Long non‐coding RNA (lncRNA) ATB belongs to an active modulator in multiple cancers, but its expression along with potential underlying non‐small cell lung cancer (NSCLC) is obscure. Our study aimed to investigate the role and potential mechanism of LncRNA ATB in NSCLC. METHODS: LncRNA ATB expression in NSCLC tissues and cell lines was detected by qRT‐PCR. Effects of LncRNA ATB on NSCLC cell proliferation, migration and invasion were assessed by MTS, colony formation and transwell assays. The connection among LncRNA ATB, miR‐200b and fibronectin 1 (FN1) was determined by bioformatics prediction and luciferase reporter assay. RESULTS: In this research, upregulation of LncRNA ATB was discovered in NSCLC tissue samples and cell lines. LncRNA ATB was positively related to advanced tumor phase as well as lymph node metastasis. Cell function assays reflected LncRNA ATB expedited NSCLC cells proliferation, migration and invasion. LncRNA ATB promoted fibronectin 1 (FN1) expression via inhibiting miR‐200b. Furthermore, LncRNA ATB depletion suppressed NSCLC cells proliferation, migration and invasion, while miR‐200b inhibitor or pcDNA‐FN1 rescued these effects. CONCLUSION: In summary, our outcomes elucidated that LncRNA ATB/miR‐200b axis expedited NSCLC cells proliferation, migration and invasion by up‐regulating FN1. |
format | Online Article Text |
id | pubmed-10020841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100208412023-03-18 Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis Sun, Shifang Zou, Yifan Xu, Ningjie Wang, Kaiyue Rong, Shanshan Lv, Jiarong Hu, Bin Mai, Yifeng Zhu, Decai Ding, Liren J Clin Lab Anal Research Articles BACKGROUND: Long non‐coding RNA (lncRNA) ATB belongs to an active modulator in multiple cancers, but its expression along with potential underlying non‐small cell lung cancer (NSCLC) is obscure. Our study aimed to investigate the role and potential mechanism of LncRNA ATB in NSCLC. METHODS: LncRNA ATB expression in NSCLC tissues and cell lines was detected by qRT‐PCR. Effects of LncRNA ATB on NSCLC cell proliferation, migration and invasion were assessed by MTS, colony formation and transwell assays. The connection among LncRNA ATB, miR‐200b and fibronectin 1 (FN1) was determined by bioformatics prediction and luciferase reporter assay. RESULTS: In this research, upregulation of LncRNA ATB was discovered in NSCLC tissue samples and cell lines. LncRNA ATB was positively related to advanced tumor phase as well as lymph node metastasis. Cell function assays reflected LncRNA ATB expedited NSCLC cells proliferation, migration and invasion. LncRNA ATB promoted fibronectin 1 (FN1) expression via inhibiting miR‐200b. Furthermore, LncRNA ATB depletion suppressed NSCLC cells proliferation, migration and invasion, while miR‐200b inhibitor or pcDNA‐FN1 rescued these effects. CONCLUSION: In summary, our outcomes elucidated that LncRNA ATB/miR‐200b axis expedited NSCLC cells proliferation, migration and invasion by up‐regulating FN1. John Wiley and Sons Inc. 2023-02-20 /pmc/articles/PMC10020841/ /pubmed/36806318 http://dx.doi.org/10.1002/jcla.24822 Text en © 2023 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Sun, Shifang Zou, Yifan Xu, Ningjie Wang, Kaiyue Rong, Shanshan Lv, Jiarong Hu, Bin Mai, Yifeng Zhu, Decai Ding, Liren Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title | Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title_full | Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title_fullStr | Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title_full_unstemmed | Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title_short | Long non‐coding RNA ATB expedites non‐small cell lung cancer progression by the miR‐200b/fibronectin 1 axis |
title_sort | long non‐coding rna atb expedites non‐small cell lung cancer progression by the mir‐200b/fibronectin 1 axis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10020841/ https://www.ncbi.nlm.nih.gov/pubmed/36806318 http://dx.doi.org/10.1002/jcla.24822 |
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