Protocol to establish a genetically tractable synthetic symbiosis between Sodalis praecaptivus and grain weevils by insect egg microinjection

We present a protocol to establish a synthetic symbiosis between the mCherry-expressing Sodalis praecaptivus and the grain weevil host, Sitophilus zeamais. We describe steps to isolate grain weevil eggs, followed by microinjecting the bacterial symbiont into insect eggs using a modified Drosophila i...

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Detalles Bibliográficos
Autores principales: Su, Yinghua, Lin, Ho-Chen, Dale, Colin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025269/
https://www.ncbi.nlm.nih.gov/pubmed/36917608
http://dx.doi.org/10.1016/j.xpro.2023.102156

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025269/
https://www.ncbi.nlm.nih.gov/pubmed/36917608
http://dx.doi.org/10.1016/j.xpro.2023.102156

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