Cargando…

Protocol for in vitro phosphorylation of the MUS81-binding region of SLX4 using CDK1-cyclin B

Phosphorylation is a post-translational modification that can alter protein structure and regulate protein-protein interactions. Here, we present a procedure for in vitro phosphorylation of the MUS81-binding region of SLX4 (SLX4(MBR)) using cyclin-dependent kinase 1-cyclin B. We describe steps for t...

Descripción completa

Detalles Bibliográficos
Autores principales: Payliss, Brandon J., Wyatt, Haley D.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025271/
https://www.ncbi.nlm.nih.gov/pubmed/36917604
http://dx.doi.org/10.1016/j.xpro.2023.102152