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Protocol for in vitro phosphorylation of the MUS81-binding region of SLX4 using CDK1-cyclin B
Phosphorylation is a post-translational modification that can alter protein structure and regulate protein-protein interactions. Here, we present a procedure for in vitro phosphorylation of the MUS81-binding region of SLX4 (SLX4(MBR)) using cyclin-dependent kinase 1-cyclin B. We describe steps for t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10025271/ https://www.ncbi.nlm.nih.gov/pubmed/36917604 http://dx.doi.org/10.1016/j.xpro.2023.102152 |