Direct whole-cell patch-clamp recordings from small boutons in rodent primary neocortical neuron cultures

Direct electrical recordings from conventional boutons in the mammalian central nervous system have proven challenging due to their small size. Here, we provide a protocol for direct whole-cell patch-clamp recordings from small presynaptic boutons of primary dissociated cultured neurons of the roden...

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Detalles Bibliográficos
Autores principales: Ritzau-Jost, Andreas, Nerlich, Jana, Kaas, Thomas, Krueger, Martin, Tsintsadze, Timur, Eilers, Jens, Barbour, Boris, Smith, Stephen M., Hallermann, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026040/
https://www.ncbi.nlm.nih.gov/pubmed/36920913
http://dx.doi.org/10.1016/j.xpro.2023.102168

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10026040/
https://www.ncbi.nlm.nih.gov/pubmed/36920913
http://dx.doi.org/10.1016/j.xpro.2023.102168

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