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Single-cell Proteomics Preparation for Mass Spectrometry Analysis using Freeze-Heat Lysis and an Isobaric Carrier

Single-cell proteomics analysis requires sensitive, quantitatively accurate, widely accessible, and robust methods. To meet these requirements, the Single-Cell ProtEomics (SCoPE2) protocol was developed as a second-generation method for quantifying hundreds to thousands of proteins from limited samp...

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Detalles Bibliográficos
Autores principales: Petelski, Aleksandra A., Slavov, Nikolai, Specht, Harrison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027359/
https://www.ncbi.nlm.nih.gov/pubmed/36571403
http://dx.doi.org/10.3791/63802